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PMID: 8306972 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

MSG5, a novel protein phosphatase promotes adaptation to pheromone response in S. cerevisiae.

The EMBO journal ·Vol. 13 ·No. 1 ·1994-01-01 ·Pages 61-70

Doi K, Gartner A, Ammerer G, Errede B, Shinkawa H, Sugimoto K, Matsumoto K

Abstract

Pheromone-stimulated yeast cells and haploid gpa1 deletion mutants arrest their cell cycle in G1. Overexpression of a novel gene called MSG5 suppresses this inhibition of cell division. Loss of MSG5 function leads to a diminished adaptive response to pheromone. Genetic analysis indicates that MSG5 acts at a stage where the protein kinases STE7 and FUS3 function to transmit the pheromone-induced signal. Since loss of MSG5 function causes an increase in FUS3 enzyme activity but not STE7 activity, we propose that MSG5 impinges on the pathway at FUS3. Sequence analysis suggests that MSG5 encodes a protein tyrosine phosphatase. This is supported by the finding that recombinant MSG5 has phosphatase activity in vitro and is able to inactivate autophosphorylated FUS3. Thus MSG5 might stimulate recovery from pheromone by regulating the phosphorylation state of FUS3.

Related Genes
MSG5
MeSH Terms
Adaptation, Physiological/genetics Amino Acid Sequence Base Sequence Binding Sites DNA, Fungal Fungal Proteins/genetics,metabolism Gene Expression Regulation, Enzymologic Gene Expression Regulation, Fungal Genes, Fungal Molecular Sequence Data Mutation Pheromones/physiology Plasmids Protein Tyrosine Phosphatases/genetics,metabolism Saccharomyces cerevisiae/enzymology,genetics Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Signal Transduction
Chemicals
DNA, Fungal Fungal Proteins Pheromones Saccharomyces cerevisiae Proteins MSG5 protein, S cerevisiae Protein Tyrosine Phosphatases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Doi K
Department of Molecular Biology, Faculty of Science, Nagoya University, Japan.
Gartner A
Ammerer G
Errede B
Shinkawa H
Sugimoto K
Matsumoto K
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-01-01
Pages
61-70
Language
English
Region
England
NLM ID
8208664
PMCID
PMC394779
Subset
IM
Grants
NIGMS NIH HHS · GM-30619 · United States
NIGMS NIH HHS · GM-39582 · United States
Databases
GENBANK
D17548
External Links
PubMed source PMC Full Text View journal details
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