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PMID: 8455599 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Pheromone-induced signal transduction in Saccharomyces cerevisiae requires the sequential function of three protein kinases.

Molecular and cellular biology ·Vol. 13 ·No. 4 ·1993-04-00 ·Pages 2069-80

Zhou Z, Gartner A, Cade R, Ammerer G, Errede B

Abstract

Protein phosphorylation plays an important role in pheromone-induced differentiation processes of haploid yeast cells. Among the components necessary for signal transduction are the STE7 and STE11 kinases and either one of the redundant FUS3 and KSS1 kinases. FUS3 and presumably KSS1 are phosphorylated and activated during pheromone induction by a STE7-dependent mechanism. Pheromone also induces the accumulation of STE7 in a hyperphosphorylated form. This modification of STE7 requires the STE11 kinase, which is proposed to act before STE7 during signal transmission. Surprisingly, STE7 hyperphosphorylation also requires a functional FUS3 (or KSS1) kinase. Using in vitro assays for FUS3 phosphorylation, we show that pheromone activates STE7 even in the absence of FUS3 and KSS1. Therefore, STE7 activation must precede modification of FUS3 (and KSS1). These findings suggest that STE7 hyperphosphorylation is a consequence of its activation but not the determining event.

Related Genes
FUS3 STE11 STE7
MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Western Cloning, Molecular Fungal Proteins/metabolism MAP Kinase Kinase Kinases Mating Factor Mitogen-Activated Protein Kinase Kinases Mitogen-Activated Protein Kinases Molecular Sequence Data Oligodeoxyribonucleotides/chemistry Peptides/physiology Pheromones/physiology Phosphoproteins/metabolism Phosphorylation Protein Kinases/metabolism Protein-Tyrosine Kinases/metabolism Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins Signal Transduction
Chemicals
Fungal Proteins Oligodeoxyribonucleotides Peptides Pheromones Phosphoproteins Saccharomyces cerevisiae Proteins Mating Factor Protein Kinases Protein-Tyrosine Kinases FUS3 protein, S cerevisiae KSS1 protein, S cerevisiae Mitogen-Activated Protein Kinases MAP Kinase Kinase Kinases Ste11 protein, S cerevisiae Mitogen-Activated Protein Kinase Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Zhou Z
Department of Chemistry, University of North Carolina, Chapel Hill 27599.
Gartner A
Cade R
Ammerer G
Errede B
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47 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.4.2069-2080.1993
Published
1993-04-00
Pages
2069-80
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359528
Subset
IM
Grants
NIGMS NIH HHS · GM-30619 · United States
NIGMS NIH HHS · GM-39852 · United States
External Links
PubMed source DOI: 10.1128/mcb.13.4.2069-2080.1993 PMC Full Text View journal details
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