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PMID: 8321216 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA replication of histone gene repeats in Drosophila melanogaster tissue culture cells: multiple initiation sites and replication pause sites.

Molecular and cellular biology ·Vol. 13 ·No. 7 ·1993-07-00 ·Pages 4098-106

Shinomiya T, Ina S

Abstract

We showed previously that DNA replication initiates at multiple sites in the 5-kb histone gene repeating unit in early embryos of Drosophila melanogaster. The present report shows evidence that replication in the same chromosomal region initiates at multiple sites in tissue culture cells as well. First, we analyzed replication intermediates by the two-dimensional gel electrophoretic replicon mapping method and detected bubble-form replication intermediates for all fragments restricted at different sites in the repeating unit. Second, we analyzed bromodeoxyuridine-labeled nascent strands amplified by the polymerase chain reaction method and detected little differences in the size distribution of nascent strands specific to six short segments located at different sites in the repeating unit. These results strongly suggest that DNA replication initiates at multiple sites located within the repeating unit. We also found several replication pause sites located at 5' upstream regions of some histone genes.

MeSH Terms
Animals Bromodeoxyuridine Cells, Cultured DNA Replication Drosophila melanogaster/cytology,genetics Electrophoresis, Gel, Two-Dimensional Histones/genetics Polymerase Chain Reaction Repetitive Sequences, Nucleic Acid Restriction Mapping
Chemicals
Histones Bromodeoxyuridine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Shinomiya T
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Ina S
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.7.4098-4106.1993
Published
1993-07-00
Pages
4098-106
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359959
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.13.7.4098-4106.1993 PMC Full Text View journal details
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