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PMID: 8327478 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Association of the high-affinity receptor for IgG (Fc gamma RI) with the gamma subunit of the IgE receptor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 13 ·1993-07-01 ·Pages 6023-7

Ernst LK, Duchemin AM, Anderson CL

Abstract

How receptors for the Fc portion of IgG antibodies (Fc gamma R) trigger a variety of immune functions by clustering upon engagement of ligand is largely unknown. Of the three distinct classes of human Fc gamma R, only Fc gamma RIIIA has been shown to associate with a potential signal-generating subunit, either the gamma chain or the zeta chain. With these studies we show that Fc gamma RI, the high-affinity receptor for IgG, also is found in association with gamma chain. This association can be demonstrated by using anti-Fc gamma RI antibodies, Fc gamma RI ligand, and anti-gamma-chain antibodies that coadsorb the proteins from detergent lysates of Fc gamma RI-expressing cells. The association of Fc gamma RI and gamma chain can be reconstituted by cotransfection of cDNAs for both proteins into COS cells.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Base Sequence Blotting, Western Cell Line Humans Interferon-gamma/pharmacology Molecular Sequence Data Polymerase Chain Reaction RNA, Messenger/analysis Receptors, IgE/analysis,genetics,immunology Receptors, IgG/analysis,genetics,immunology Transfection Tumor Cells, Cultured
Chemicals
Antibodies, Monoclonal RNA, Messenger Receptors, IgE Receptors, IgG Interferon-gamma
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ernst L K
Department of Internal Medicine, Ohio State University, Columbus 43210.
Duchemin A M
Anderson C L
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31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.13.6023
Published
1993-07-01
Pages
6023-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC46859
Subset
IM
Grants
NCI NIH HHS · R01-CA44983 · United States
External Links
PubMed source DOI: 10.1073/pnas.90.13.6023 PMC Full Text View journal details
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