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PMID: 8346014 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A role in DNA binding for the linker sequences of the first three zinc fingers of TFIIIA.

Nucleic acids research ·Vol. 21 ·No. 15 ·1993-07-25 ·Pages 3341-6

Choo Y, Klug A

Abstract

Zinc fingers of the TFIIIA type are connected by short linker sequences between the structural units. Structural investigations by 2D NMR in solution and by X-ray crystallographic analyses of complexes with DNA point to a passive role for the linkers. We have therefore investigated the influence of the linker sequence on DNA binding using as a model the first three fingers of the protein TFIIIA. Insertion of certain heterologous linkers abolishes binding, and replacement of individual amino acids can reduce binding by factors of up to twenty-four.

Related Genes

TF321 TF3A TF43A TF43B

MeSH Terms

Amino Acid Sequence Binding Sites Cloning, Molecular DNA/metabolism Magnetic Resonance Spectroscopy Molecular Sequence Data Mutagenesis, Site-Directed Protein Biosynthesis Structure-Activity Relationship Transcription Factor TFIIIA Transcription Factors/chemistry,genetics,metabolism Transcription, Genetic X-Ray Diffraction Zinc Fingers

Chemicals

Transcription Factor TFIIIA Transcription Factors DNA

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Choo Y

Medical Research Council, Laboratory of Molecular Biology, Cambridge, UK.

Klug A

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24 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/21.15.3341

Published

1993-07-25

Pages

3341-6

Language

English

Region

England

NLM ID

0411011

PMCID

PMC331429

Subset

External Links

PubMed source DOI: 10.1093/nar/21.15.3341 PMC Full Text View journal details

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A role in DNA binding for the linker sequences of the first three zinc fingers of TFIIIA.

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