Routine application of high-performance liquid chromatography for identification of mycobacteria.
Mycolic acid analysis by high-performance liquid chromatography (HPLC) was introduced in our laboratory as the routine technique for identifying all clinical isolates of mycobacteria referred to us. HPLC identified 96.1% of the 1,103 strains analyzed, whereas the biochemical procedures and/or the commercial DNA probes identified 98.3% of strains, for an overall agreement of 94.4%. Compared with the probes, there was 100% specificity and 98.9% sensitivity for Mycobacterium tuberculosis identification. HPLC allowed early detection and identification of the rare mycobacterial species M. haemophilum, M. malmoense, M. shimoidei, and M. fallax as well as uncharacteristic strains of M. simiae. After 18 months of routine use, HPLC proved to be reliable, easy to perform, rapid, and less costly than other identification methods.
14 references, click to expand
-
Identification of clinically significant Mycobacterium fortuitum complex isolates.J Clin Microbiol. 1981 Dec;14(6):686-91 PMID: 7037841
-
[Taxonomic value of mycobacterial fatty acids: proposal for a method of analysis].Ann Microbiol (Paris). 1983 Sep-Oct;134B(2):241-56 PMID: 6651121
-
High-performance liquid chromatography of mycolic acids as a tool in the identification of Corynebacterium, Nocardia, Rhodococcus, and Mycobacterium species.J Clin Microbiol. 1986 Jan;23(1):182-5 PMID: 3700601
-
Mycolic acid analysis for clinical identification of Mycobacterium avium and related mycobacteria.J Clin Microbiol. 1986 Nov;24(5):835-9 PMID: 3771768
-
High-performance liquid chromatography analysis of mycolic acids as an aid in laboratory identification of Rhodococcus and Nocardia species.J Clin Microbiol. 1987 Nov;25(11):2126-31 PMID: 3693543
-
Identification of major slowly growing pathogenic mycobacteria and Mycobacterium gordonae by high-performance liquid chromatography of their mycolic acids.J Clin Microbiol. 1988 Jan;26(1):50-3 PMID: 3125214
-
High-performance liquid chromatography patterns of Mycobacterium gordonae mycolic acids.J Clin Microbiol. 1992 Sep;30(9):2402-7 PMID: 1401006
-
Chronic tenosynovitis of the hand due to Mycobacterium nonchromogenicum: use of high-performance liquid chromatography for identification of isolates.Rev Infect Dis. 1991 Sep-Oct;13(5):857-64 PMID: 1962098
-
Fourth report of the cooperative, open-ended study of slowly growing mycobacteria by the International Working Group on Mycobacterial Taxonomy.Int J Syst Bacteriol. 1991 Oct;41(4):463-72 PMID: 1742195
-
Identification of mycobacteria by high-performance liquid chromatography.J Clin Microbiol. 1991 Nov;29(11):2468-72 PMID: 1774251
-
Proposal of Mycobacterium peregrinum sp. nov., nom. rev., and elevation of Mycobacterium chelonae subsp. abscessus (Kubica et al.) to species status: Mycobacterium abscessus comb. nov.Int J Syst Bacteriol. 1992 Apr;42(2):240-5 PMID: 1581184
-
Separation of Mycobacterium bovis BCG from Mycobacterium tuberculosis and Mycobacterium bovis by using high-performance liquid chromatography of mycolic acids.J Clin Microbiol. 1992 May;30(5):1327-30 PMID: 1583141
-
Identification of Mycobacterium avium complex strains and some similar species by high-performance liquid chromatography.J Clin Microbiol. 1992 Oct;30(10):2698-704 PMID: 1400970
-
High-performance liquid chromatography patterns of mycolic acids as criteria for identification of Mycobacterium chelonae, Mycobacterium fortuitum, and Mycobacterium smegmatis.J Clin Microbiol. 1990 Sep;28(9):2094-8 PMID: 2229390
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
