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PMID: 8371985 Published · ppublish English Journal Article

A 127 kDa component of a UV-damaged DNA-binding complex, which is defective in some xeroderma pigmentosum group E patients, is homologous to a slime mold protein.

Nucleic acids research ·Vol. 21 ·No. 17 ·1993-08-25 ·Pages 4111-8

Takao M, Abramic M, Moos M, Otrin VR, Wootton JC, McLenigan M, Levine AS, Protic M

Abstract

A cDNA which encodes a approximately 127 kDa UV-damaged DNA-binding (UV-DDB) protein with high affinity for (6-4)pyrimidine dimers [Abramic', M., Levine, A.S. & Protic', M., J. Biol. Chem. 266: 22493-22500, 1991] has been isolated from a monkey cell cDNA library. The presence of this protein in complexes bound to UV-damaged DNA was confirmed by immunoblotting. The human cognate of the UV-DDB gene was localized to chromosome 11. UV-DDB mRNA was expressed in all human tissues examined, including cells from two patients with xeroderma pigmentosum (group E) that are deficient in UV-DDB activity, which suggests that the binding defect in these cells may reside in a dysfunctional UV-DDB protein. Database searches have revealed significant homology of the UV-DDB protein sequence with partial sequences of yet uncharacterized proteins from Dictyostelium discoideum (44% identity over 529 amino acids) and Oryza sativa (54% identity over 74 residues). According to our results, the UV-DDB polypeptide belongs to a highly conserved, structurally novel family of proteins that may be involved in the early steps of the UV response, e.g., DNA damage recognition.

Related Genes
UV-DDB
MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Chlorocebus aethiops Chromosome Mapping DNA/metabolism,radiation effects DNA Damage DNA-Binding Proteins/genetics,metabolism Dictyostelium Electrophoresis, Polyacrylamide Gel Fungal Proteins/genetics Humans Molecular Sequence Data Oryza Plant Proteins/genetics Sequence Homology, Amino Acid Ultraviolet Rays Xeroderma Pigmentosum/genetics
Chemicals
DNA-Binding Proteins Fungal Proteins Plant Proteins DNA
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Takao M
Section on DNA Replication, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.
Abramic M
Moos M
Otrin V R
Wootton J C
McLenigan M
Levine A S
Protic M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.17.4111
Published
1993-08-25
Pages
4111-8
Language
English
Region
England
NLM ID
0411011
PMCID
PMC310015
Subset
IM
Databases
GENBANK
L20216
External Links
PubMed source DOI: 10.1093/nar/21.17.4111 PMC Full Text View journal details
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