DNA homology requirements for mitotic gap repair in Drosophila.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 4 ·1993-02-15 ·Pages 1262-6

Nassif N, Engels W

Abstract

We used P transposable-element mobilization to study the repair of double-strand DNA breaks in Drosophila melanogaster premeiotic germ cells. Distribution of conversion tracts was found to be largely unaffected by changes in the length of sequence homology between the broken ends and the template, suggesting that only a short match is required. However, the frequency of repair was highly sensitive to single-base mismatches within the homologous region, ranging from 19% reversion when there were no mismatches to 5% when 15 mismatches were present over a 3455-bp span.

MeSH Terms

Alleles Animals Crosses, Genetic DNA Damage DNA Repair DNA Transposable Elements Drosophila melanogaster/genetics Female Gene Conversion Genotype Heterozygote Male Mitosis/genetics Sequence Homology, Nucleic Acid X Chromosome

Chemicals

DNA Transposable Elements

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Nassif N

Genetics Department, University of Wisconsin, Madison 53706.

Engels W

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29 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.90.4.1262

Published

1993-02-15

Pages

1262-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC45852

Subset

External Links

PubMed source DOI: 10.1073/pnas.90.4.1262 PMC Full Text View journal details

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DNA homology requirements for mitotic gap repair in Drosophila.

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