Transformation of the oomycete pathogen Phytophthora megasperma f. sp. glycinea occurs by DNA integration into single or multiple chromosomes.
A procedure for stable transformation was developed for Phytophthora megasperma f. sp. glycinea, an oomycete pathogen of soybean. Transformants were obtained using a bacterial hygromycin resistance gene fused to a promoter and terminator from the ham34 gene of another oomycete, Bremia lactucae. Vector DNA, alone or complexed to cationic liposomes, was introduced into protoplasts using polyethylene glycol and CaCl2. DNA and RNA hybridization, and phosphotransferase assays, confirmed the presence and expression of vector DNA in the transformants. Hybridization to electrophoretically separated chromosomes of P. m. glycinea showed that vector DNA had integrated into only one chromosome in four transformants, and into multiple chromosomes in one transformant.
16 references, click to expand
-
Regulatory sequences for expressing genes in oomycete fungi.Mol Gen Genet. 1992 Jul;234(1):138-46 PMID: 1495476
-
Transformation of Aspergillus nidulans by using a trpC plasmid.Proc Natl Acad Sci U S A. 1984 Mar;81(5):1470-4 PMID: 6324193
-
Cis-acting and trans-acting regulatory mutations define two types of promoters controlled by the qa-1F gene of Neurospora.Cell. 1984 Feb;36(2):493-502 PMID: 6198093
-
Efficient cloning of genes of Neurospora crassa.Proc Natl Acad Sci U S A. 1986 Jul;83(13):4869-73 PMID: 16593723
-
Cotransformation of Aspergillus nidulans: a tool for replacing fungal genes.Mol Gen Genet. 1987 Aug;209(1):71-7 PMID: 3312958
-
Separation of large DNA molecules by contour-clamped homogeneous electric fields.Science. 1986 Dec 19;234(4783):1582-5 PMID: 3538420
-
Genetic transformation of the fungal pathogen responsible for rice blast disease.Proc Natl Acad Sci U S A. 1987 Jun;84(12):4161-5 PMID: 16593854
-
Genetic transformation of the plant pathogens Phytophthora capsici and Phytophthora parasitica.Nucleic Acids Res. 1991 Aug 11;19(15):4273-8 PMID: 1651483
-
Isolation and characterization of an autonomously replicating sequence from Ustilago maydis.Mol Cell Biol. 1988 Sep;8(9):3703-9 PMID: 2851726
-
Recombinant DNA in filamentous fungi: progress and prospects.Crit Rev Biotechnol. 1987;6(4):357-93 PMID: 3333340
-
New concepts of kingdoms or organisms. Evolutionary relations are better represented by new classifications than by the traditional two kingdoms.Science. 1969 Jan 10;163(3863):150-60 PMID: 5762760
-
Nonreciprocal exchanges of information between DNA duplexes coinjected into mammalian cell nuclei.Mol Cell Biol. 1985 Jan;5(1):59-69 PMID: 2984556
-
Gene transfer system for the phytopathogenic fungus Ustilago maydis.Proc Natl Acad Sci U S A. 1988 Feb;85(3):865-9 PMID: 2829206
-
Transformation of the oomycete pathogen, Phytophthora infestans.Mol Plant Microbe Interact. 1991 Nov-Dec;4(6):602-7 PMID: 1804404
-
Transformation in fungi.Microbiol Rev. 1989 Mar;53(1):148-70 PMID: 2651864
-
Yeast transformation: a model system for the study of recombination.Proc Natl Acad Sci U S A. 1981 Oct;78(10):6354-8 PMID: 6273866
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