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PMID: 8386705 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Resistance to nitric oxide in Mycobacterium avium complex and its implication in pathogenesis.

Infection and immunity ·Vol. 61 ·No. 5 ·1993-05-00 ·Pages 1980-9

Doi T, Ando M, Akaike T, Suga M, Sato K, Maeda H

Abstract

Susceptibility of three different strains of Mycobacterium avium complex (MAC), i.e., one strain of M. avium (Mino) and two strains of M. intracellulare (31F093T and KUMS 9007), to nitric oxide (NO) generated by rat alveolar macrophages (M phi) or NO generated chemically by acidification of NO2- was examined in vitro. We also investigated the effects of NO on phagocytosis and superoxide anion (O2-) generation by M phi. The intracellular growth of M. avium Mino was significantly suppressed by NO generated by gamma interferon (IFN-gamma)-stimulated M phi, whereas that of two strains of M. intracellulare (31F093T and KUMS 9007) was not. M. avium Mino was also more susceptible to NO generated chemically by acidification of NO2- than the two M. intracellulare strains. In L-arginine (1 mM)-containing medium, NO release from the M phi assessed by measuring NO2- increased as the concentration of IFN-gamma increased. The enhancing potential of IFN-gamma for NO release became more pronounced when M phi were infected with 31F093T, an NO-resistant strain. A large amount of NO generated by IFN-gamma-stimulated M phi suppressed both phagocytosis and O2- generation by the M phi, especially after infection of the M phi with strain 31F093T. These results indicate that the intracellular growth of MAC is not always inhibited by NO generated by immunologically activated M phi; rather, NO generation induced by infection with an NO-resistant MAC strain suppresses phagocytosis of the M phi, which may allow extracellular spreading of such NO-resistant mycobacteria. Therefore, the pathogenic potential of MAC may be partly attributed to its resistance to NO.

MeSH Terms
Animals Arginine/analogs & derivatives,pharmacology Drug Resistance, Microbial Hydrogen-Ion Concentration Immunity, Cellular Interferon-gamma/pharmacology Macrophage Activation Macrophages, Alveolar/immunology,microbiology Male Mycobacterium avium/drug effects,pathogenicity Nitric Oxide/pharmacology Nitrites/pharmacology Phagocytosis/drug effects Rats Rats, Inbred F344 Rats, Wistar Recombinant Proteins Respiratory Burst/drug effects Superoxides/metabolism Tetradecanoylphorbol Acetate/pharmacology Tuberculosis/immunology omega-N-Methylarginine
Chemicals
Nitrites Recombinant Proteins Superoxides omega-N-Methylarginine Nitric Oxide Interferon-gamma Arginine Tetradecanoylphorbol Acetate
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Doi T
Department of Microbiology, Kumamoto University School of Medicine, Japan.
Ando M
Akaike T
Suga M
Sato K
Maeda H
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.61.5.1980-1989.1993
Published
1993-05-00
Pages
1980-9
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC280792
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.61.5.1980-1989.1993 PMC Full Text View journal details
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