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PMID: 8388095 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Processing of recombination intermediates by the RecG and RuvAB proteins of Escherichia coli.

Nucleic acids research ·Vol. 21 ·No. 8 ·1993-04-25 ·Pages 1719-25

Lloyd RG, Sharples GJ

Abstract

The RuvAB, RuvC and RecG proteins of Escherichia coli process intermediates in recombination and DNA repair into mature products. RuvAB and RecG catalyse branch migration of Holliday junctions, while RuvC resolves these structures by nuclease cleavage around the point of strand exchange. The overlap between RuvAB and RecG was investigated using synthetic X- and Y-junctions. RuvAB is a complex of RuvA and RuvB, with RuvA providing the DNA binding subunit and RuvB the ATPase activity that drives branch migration. Both RuvA and RecG form defined complexes with each of the junctions. The gel mobilities of these complexes suggests that the X-junction attracts two tetramers of RuvA, but mainly monomers of RecG. Dissociation of the junction in the presence of ATP requires high levels of RuvAB. RecG is shown to have a much higher specific activity to the extent that very little of this protein would be required to match RuvAB in vivo. Both proteins also dissociate a Y-junction, which is consistent with helicase activity. However, RecG shows no ability to unwind more conventional substrates and the suggestion is made that its helicase activity is directed towards specific DNA structures such as junctions.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/metabolism Base Sequence DNA Helicases/metabolism DNA Repair DNA, Bacterial/metabolism DNA-Binding Proteins/metabolism Escherichia coli/genetics,metabolism Escherichia coli Proteins Kinetics Molecular Sequence Data Protein Binding Recombination, Genetic Sequence Homology, Amino Acid
Chemicals
Bacterial Proteins DNA, Bacterial DNA-Binding Proteins Escherichia coli Proteins RuvB protein, Bacteria RecG protein, E coli Holliday junction DNA helicase, E coli DNA Helicases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lloyd R G
Department of Genetics, University of Nottingham, Queens Medical Centre, UK.
Sharples G J
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.8.1719
Published
1993-04-25
Pages
1719-25
Language
English
Region
England
NLM ID
0411011
PMCID
PMC309406
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1093/nar/21.8.1719 PMC Full Text View journal details
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