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PMID: 8391057 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Analysis of T cell antigen receptor (TCR) expression by human peripheral blood CD4-8- alpha/beta T cells demonstrates preferential use of several V beta genes and an invariant TCR alpha chain.

The Journal of experimental medicine ·Vol. 178 ·No. 1 ·1993-07-01 ·Pages 1-16

Porcelli S, Yockey CE, Brenner MB, Balk SP

Abstract

CD4-CD8- (double negative [DN]) alpha/beta T cells are a largely uncharacterized subpopulation of unknown function. To investigate whether these cells are selected to recognize particular antigens or antigen-presenting molecules, DN alpha/beta T cells were purified from the peripheral blood of five normal donors and their T cell receptor (TCR) alpha and beta chains were examined. Random cloning of TCR alpha chains by single-sided polymerase chain reaction (PCR) amplification identified an invariant rearrangement between V alpha 24 and J alpha Q, with no N region diversity, which was expressed preferentially by DN alpha/beta T cells from all donors. Random cloning also identified a precise V alpha 7.2-J alpha (IGRJa14) rearrangement, with two variable amino acids encoded in the V-J junction, which was enriched in the DN alpha/beta T cell preparations from some, but not all, donors. Analysis of TCR beta chains by quantitative PCR amplification demonstrated that the expression of four V beta gene families, V beta 2, 8, 11, and 13, was markedly increased in these DN alpha/beta T cell preparations. The expression of particular TCRs by DN alpha/beta T cells from multiple donors indicates that these cells, or at least a subpopulation of cells with this phenotype, recognize a limited spectrum of antigens and suggests that they may use nonpolymorphic antigen-presenting molecules.

MeSH Terms
Amino Acid Sequence Animals Base Sequence CD4 Antigens/analysis CD8 Antigens/analysis Cells, Cultured Cloning, Molecular Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor Gene Rearrangement, beta-Chain T-Cell Antigen Receptor Humans Mice Molecular Sequence Data Polymerase Chain Reaction RNA, Messenger/analysis Receptors, Antigen, T-Cell, alpha-beta/analysis,genetics T-Lymphocytes/immunology
Chemicals
CD4 Antigens CD8 Antigens RNA, Messenger Receptors, Antigen, T-Cell, alpha-beta
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Porcelli S
Department of Rheumatology/Immunology, Brigham and Women's Hospital, Boston, Massachusetts 02115.
Yockey C E
Brenner M B
Balk S P
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.178.1.1
Published
1993-07-01
Pages
1-16
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2191070
Subset
IM
Grants
NIAID NIH HHS · AI-28973 · United States
Corrections
CommentIn
PMID: 25049428
External Links
PubMed source DOI: 10.1084/jem.178.1.1 PMC Full Text View journal details
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