Home LiteratureArticle Details
PMID: 2998758 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

DO beta: a new beta chain gene in HLA-D with a distinct regulation of expression.

The EMBO journal ·Vol. 4 ·No. 11 ·1985-11-00 ·Pages 2839-47

Tonnelle C, DeMars R, Long EO

Abstract

The HLA-D region of the human major histocompatibility complex encodes the genes for the alpha and beta chains of the DP, DQ and DR class II antigens. A cDNA clone encoding a new class II beta chain (designated DO) was isolated from a library constructed from mRNA of a mutant B-cell line having a single HLA haplotype. Complete cDNA clones encoding the four isotypic beta chains of the DR1, DQw1, DPw2 and putative DO antigens were sequenced. The DO beta gene was mapped in the D region by hybridization with DNA of HLA-deletion mutants. DO beta mRNA expression is low in B-cell lines but remains in mutant lines which have lost expression of other class II genes. Unlike other class II genes DO beta is not induced by gamma-interferon in fibroblast lines. The DO beta gene is distinct from the DP beta, DQ beta and DR beta genes in its pattern of nucleotide divergence. The independent evolution and expression of DO beta suggest that it may be part of a functionally distinct class II molecule.

MeSH Terms
Amino Acid Sequence B-Lymphocytes/immunology Base Sequence Cell Line Cloning, Molecular DNA/analysis DNA Restriction Enzymes Genes Genes, MHC Class II Genes, Regulator Genetic Variation Histocompatibility Antigens Class II/analysis Humans Macromolecular Substances Major Histocompatibility Complex Mutation
Chemicals
Histocompatibility Antigens Class II Macromolecular Substances DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tonnelle C
DeMars R
Long E O
References (60)
60 references, click to expand
  1. The evolution of genes: the chicken preproinsulin gene.
    Cell. 1980 Jun;20(2):555-66 PMID: 7388949
  2. Structure, sequence and polymorphism in the HLA-D region.
    Immunol Rev. 1985 Jul;85:5-43 PMID: 2995241
  3. Gamma ray-induced loss of expression of HLA and glyoxalase I alleles in lymphoblastoid cells.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):4251-5 PMID: 6933474
  4. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
  5. A rapid boiling method for the preparation of bacterial plasmids.
    Anal Biochem. 1981 Jun;114(1):193-7 PMID: 6269464
  6. High-efficiency cloning of full-length cDNA.
    Mol Cell Biol. 1982 Feb;2(2):161-70 PMID: 6287227
  7. Sequence of an HLA-DR alpha-chain cDNA clone and intron-exon organization of the corresponding gene.
    Nature. 1982 Oct 21;299(5885):750-2 PMID: 6811954
  8. Allelic polymorphism and complexity of the genes for HLA-DR beta-chains--direct analysis by DNA-DNA hybridization.
    Nature. 1982 Nov 25;300(5890):372-4 PMID: 6292729
  9. Isolation of distinct cDNA clones encoding HLA-DR beta chains by use of an expression assay.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7465-9 PMID: 6818545
  10. The structure of a mutant H-2 gene suggests that the generation of polymorphism in H-2 genes may occur by gene conversion-like events.
    Nature. 1983 Feb 24;301(5902):671-4 PMID: 6828150
  11. Human B cell variants immunoselected against a single Ia antigen subset have lost expression of several Ia antigen subsets.
    J Exp Med. 1983 Mar 1;157(3):1053-8 PMID: 6403646
  12. A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.
    Mol Cell Biol. 1983 Feb;3(2):280-9 PMID: 6300662
  13. Unusual evolutionary conservation and frequent DNA segment exchange in class I genes of the major histocompatibility complex.
    Proc Natl Acad Sci U S A. 1983 May;80(9):2671-5 PMID: 6573677
  14. Studies on transformation of Escherichia coli with plasmids.
    J Mol Biol. 1983 Jun 5;166(4):557-80 PMID: 6345791
  15. Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed.
    Mol Cell Biol. 1983 May;3(5):787-95 PMID: 6865942
  16. A minimum of four human class II alpha-chain genes are encoded in the HLA region of chromosome 6.
    Nature. 1983 Jul 14-20;304(5922):174-7 PMID: 6306475
  17. Complete sequence of an HLA-dR beta chain deduced from a cDNA clone and identification of multiple non-allelic DR beta chain genes.
    EMBO J. 1983;2(3):389-94 PMID: 11894954
  18. A membrane-filter technique for the detection of complementary DNA.
    Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
  19. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  20. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  21. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8 PMID: 73185
  22. Sequence organization of a cloned tDNA met fragment from Xenopus laevis.
    Cell. 1978 Jul;14(3):713-24 PMID: 688390
  23. MHC-restricted cytotoxic T cells: studies on the biological role of polymorphic major transplantation antigens determining T-cell restriction-specificity, function, and responsiveness.
    Adv Immunol. 1979;27:51-177 PMID: 92183
  24. Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper.
    Methods Enzymol. 1979;68:220-42 PMID: 94421
  25. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  26. Computer analysis of nucleic acids and proteins.
    Methods Enzymol. 1980;65(1):595-609 PMID: 6154871
  27. Structure of class I major histocompatibility antigens.
    Contemp Top Mol Immunol. 1983;9:1-63 PMID: 6191921
  28. Primary structure of human class II histocompatibility antigens 3rd communication. Amino acid sequence comparison between DR and DC subclass antigens derived from a lymphoblastoid B cell line homozygous at the HLA loci (HLA-A3,3; B7,7; Dw2,2; DR2,2: MT1,1; Dc1,1: MB1,1).
    Hoppe Seylers Z Physiol Chem. 1983 Jun;364(6):749-55 PMID: 6576979
  29. Complete primary structures of the E beta chain and gene of the mouse major histocompatibility complex.
    Proc Natl Acad Sci U S A. 1983 Sep;80(18):5520-4 PMID: 6310581
  30. Genetic mapping of a human class II antigen beta-chain cDNA clone to the SB region of the HLA complex.
    Proc Natl Acad Sci U S A. 1983 Oct;80(19):6036-40 PMID: 6310612
  31. T cell subsets and the recognition of MHC class.
    Immunol Rev. 1983;74:129-42 PMID: 6226585
  32. NH2-terminal sequence of the alpha and beta chains of human DC-1 antigen isolated from the JY cell line. Homology with murine I-A molecules.
    Immunogenetics. 1983;18(5):453-9 PMID: 6417007
  33. Mapping SB in relation to HLA and GLO1 using cells from first-cousin marriage offspring.
    Immunogenetics. 1983;18(5):503-12 PMID: 6417008
  34. Dissection of the D-region of the human major histocompatibility complex by means of induced mutations in a lymphoblastoid cell line.
    Hum Immunol. 1983 Oct;8(2):123-39 PMID: 6417067
  35. Exon-intron organization and complete nucleotide sequence of a human major histocompatibility antigen DC beta gene.
    Proc Natl Acad Sci U S A. 1983 Dec;80(23):7313-7 PMID: 6316358
  36. Structural polymorphism of the DC1 light chains from DR1-, DR2-, and DRw6-positive cell lines.
    Immunogenetics. 1983;18(6):625-37 PMID: 6581120
  37. Isotypic and allotypic variation of human class II histocompatibility antigen alpha-chain genes.
    Nature. 1984 Mar 22-28;308(5957):327-33 PMID: 6584734
  38. Nucleotide sequence of mutant I-A beta bm12 gene is evidence for genetic exchange between mouse immune response genes.
    Nature. 1984 Apr 5-11;308(5959):551-3 PMID: 6324001
  39. Both alpha and beta chains of HLA-DC class II histocompatibility antigens display extensive polymorphism in their amino-terminal domains.
    EMBO J. 1984 Feb;3(2):447-52 PMID: 6585297
  40. Ia-negative B-cell variants reveal a coordinate regulation in the transcription of the HLA class II gene family.
    Immunogenetics. 1984;19(4):349-53 PMID: 6609124
  41. T-cell clones with similar antigen specificity may be restricted by DR, MT(DC), or SB class II HLA molecules.
    Immunogenetics. 1984;19(5):455-60 PMID: 6427104
  42. CG dinucleotide clusters in MHC genes and in 5' demethylated genes.
    Nucleic Acids Res. 1984 May 25;12(10):4385-96 PMID: 6587321
  43. Translation and assembly of HLA-DR antigens in Xenopus oocytes injected with mRNA from a human B-cell line.
    EMBO J. 1982;1(5):649-54 PMID: 6821356
  44. Recombination within the HLA-D region. Correlation of molecular genotyping with functional data.
    J Exp Med. 1984 Jul 1;160(1):222-38 PMID: 6330268
  45. Molecular organization of the HLA-SB region of the human major histocompatibility complex and evidence for two SB beta-chain genes.
    Proc Natl Acad Sci U S A. 1984 Jul;81(13):3934-8 PMID: 6330724
  46. The nucleotide sequence of the murine I-E beta b immune response gene: evidence for gene conversion events in class II genes of the major histocompatibility complex.
    EMBO J. 1984 Jun;3(6):1221-5 PMID: 6086309
  47. Mutations and selection in the generation of class II histocompatibility antigen polymorphism.
    EMBO J. 1984 Jul;3(7):1655-61 PMID: 6589154
  48. Structural relationship of the SB beta-chain gene to HLA-D-region genes and murine I-region genes.
    Nature. 1984 Jul 19-25;310(5974):233-5 PMID: 6431294
  49. Immune interferon activates multiple class II major histocompatibility complex genes and the associated invariant chain gene in human endothelial cells and dermal fibroblasts.
    Proc Natl Acad Sci U S A. 1984 Aug;81(15):4917-21 PMID: 6431411
  50. Linkage map of two HLA-SB beta and two HLA-SB alpha-related genes: an intron in one of the SB beta genes contains a processed pseudogene.
    Cell. 1984 Aug;38(1):241-9 PMID: 6088068
  51. Cloning and sequence analysis of the human major histocompatibility complex gene DC-3 beta.
    Proc Natl Acad Sci U S A. 1984 Aug;81(16):5199-203 PMID: 6206493
  52. The Eb beta gene may have acted as the donor gene in a gene conversion-like event generating the Abm 12 beta mutant.
    EMBO J. 1984 Sep;3(9):2029-32 PMID: 6436017
  53. Homozygous deletions that simultaneously eliminate expressions of class I and class II antigens of EBV-transformed B-lymphoblastoid cells. I. Reduced proliferative responses of autologous and allogeneic T cells to mutant cells that have decreased expression of class II antigens.
    Hum Immunol. 1984 Oct;11(2):77-97 PMID: 6092300
  54. Localization of the human MHC-linked complement genes between HLA-B and HLA-DR by using HLA mutant cell lines.
    J Immunol. 1985 Jan;134(1):641-3 PMID: 3917284
  55. Structure and polymorphism of the HLA class II SB light chain genes.
    EMBO J. 1984 Dec 1;3(12):2985-93 PMID: 6098459
  56. Characterization of an HLA DR beta pseudogene.
    Proc Natl Acad Sci U S A. 1985 Mar;82(5):1475-9 PMID: 3856276
  57. Allelic polymorphism and transassociation of molecules encoded by the HLA-DQ subregion.
    Proc Natl Acad Sci U S A. 1985 Mar;82(6):1776-80 PMID: 3856859
  58. DNA sequence and characterization of human class II major histocompatibility complex beta chains from the DR1 haplotype.
    Proc Natl Acad Sci U S A. 1985 May;82(10):3405-9 PMID: 3858829
  59. Human cytotoxic T-lymphocyte recognition of an HLA-A3 gene product expressed on murine L cells: the only human gene product required on the target cells for lysis is the class I heavy chain.
    Proc Natl Acad Sci U S A. 1985 Jul;82(13):4490-4 PMID: 3892536
  60. Plasmid screening at high colony density.
    Gene. 1980 Jun;10(1):63-7 PMID: 6997135
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-11-00
Pages
2839-47
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554587
Subset
IM
Databases
GENBANK
X03066, X03067, X03068, X03069
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2