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PMID: 8393009 Published · ppublish English Journal Article

Genotypic identification and phylogenetic analysis of the spotted fever group rickettsiae by pulsed-field gel electrophoresis.

Journal of bacteriology ·Vol. 175 ·No. 15 ·1993-08-00 ·Pages 4895-904

Roux V, Raoult D

Abstract

Using pulsed-field gel electrophoresis, we studied the chromosomes of spotted fever group rickettsiae. We digested the DNA of 16 species currently known to belong to this group with SmaI, EagI, and BssHII. The genome size of 13 rickettsiae was between 1,200 and 1,300 kb. "Rickettsia massiliae" and "R. helvetica" genome sizes were 1,370 and 1,397 kb, respectively, and that of R. bellii was 1,660 kb. It was possible to obtain distinctive patterns for each species, but in R. conorii, 10 isolates exhibited the same profiles, showing that pulsed-field gel electrophoresis is a good interspecies identification tool. We achieved a phylogenetic analysis of these bacteria by using the Dice coefficient and UPGMA and Package Philip programming. We established a dendrogram of the genetic relationships between the different species showing the existence of a cluster in the spotted fever group rickettsiae including R. conorii, R. rickettsii, R. parkeri, R. sibirica, "R. africae," "R. slovaca," Thai tick typhus rickettsia, and Israeli tick typhus rickettsia. We located three genes previously cloned and sequenced (genes encoding the R. rickettsii surface proteins of 120 and 190 kDa and the R. prowazekii citrate synthase gene), using Southern hybridization. The genes encoding citrate synthase and the surface protein of 190 kDa were usually located on the same band, and it is hypothesized that they are relatively close on the chromosome.

MeSH Terms
Chromosome Mapping Chromosomes, Bacterial DNA Restriction Enzymes DNA, Bacterial/genetics Electrophoresis, Gel, Pulsed-Field Gene Amplification Genes, Bacterial/genetics Genome, Bacterial Phylogeny Rickettsia/genetics
Chemicals
DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Roux V
Unité des Rickettsies, Faculté de Médecine, Marseille, France.
Raoult D
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.15.4895-4904.1993
Published
1993-08-00
Pages
4895-904
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC204943
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.175.15.4895-4904.1993 PMC Full Text View journal details
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