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PMID: 8404854 Published · ppublish English Journal Article

Deposition of chromosomal protein HMG-17 during replication affects the nucleosomal ladder and transcriptional potential of nascent chromatin.

The EMBO journal ·Vol. 12 ·No. 10 ·1993-10-00 ·Pages 3855-64

Crippa MP, Trieschmann L, Alfonso PJ, Wolffe AP, Bustin M

Abstract

A cell-free system from Xenopus eggs was used to study the role of chromosomal protein HMG-17 in the generation of the chromatin structure of transcriptionally active genes. Addition of HMG-17 protein to the extracts, which do not contain structural homologs of the HMG-14/-17 protein family, indicates the protein is incorporated into the nascent template during replication, prior to completion of chromatin assembly. The protein binds to and stabilizes the structure of the nucleosomal core thereby improving the apparent periodicity of the nucleosomal spacing of nascent chromatin. Assembly of HMG-17 into the nascent chromatin structure significantly increased the transcription potential of the 5S RNA gene and satellite I chromatin. Kinetic studies indicate that the increase in transcriptional potential is observed only when HMG-17 is incorporated into nucleosomes during chromatin assembly.

MeSH Terms
Animals Blotting, Western Cell-Free System Chromatin/metabolism DNA Replication High Mobility Group Proteins/metabolism Nucleosomes/metabolism Oocytes Periodicity Transcription, Genetic Xenopus laevis
Chemicals
Chromatin High Mobility Group Proteins Nucleosomes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Crippa M P
Laboratory of Molecular Carcinogenesis, NCI, National Institutes of Health, Bethesda, MD 20892.
Trieschmann L
Alfonso P J
Wolffe A P
Bustin M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-10-00
Pages
3855-64
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413669
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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