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PMID: 8407793 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Characterization of the ferrous iron uptake system of Escherichia coli.

Journal of bacteriology ·Vol. 175 ·No. 19 ·1993-10-00 ·Pages 6212-9

Kammler M, Schön C, Hantke K

Abstract

Escherichia coli has an iron(II) transport system (feo) which may make an important contribution to the iron supply of the cell under anaerobic conditions. Cloning and sequencing of the iron(II) transport genes revealed an open reading frame (feoA) possibly coding for a small protein with 75 amino acids and a membrane protein with 773 amino acids (feoB). The upstream region of feoAB contained a binding site for the regulatory protein Fur, which acts with iron(II) as a corepressor in all known iron transport systems of E. coli. In addition, a Fnr binding site was identified in the promoter region. The FeoB protein had an apparent molecular mass of 70 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and was localized in the cytoplasmic membrane. The sequence revealed regions of homology to ATPases, which indicates that ferrous iron uptake may be ATP driven. FeoA or FeoB mutants could be complemented by clones with the feoA or feoB gene, respectively.

Related Genes
aerA dmsA fecA feo feoA feoB fepA fhuA fhuG frdA fur glpA iucA narG nirB sodA tonB
MeSH Terms
Adenosine Triphosphatases/genetics Aerobiosis Amino Acid Sequence Anaerobiosis Animals Base Sequence Binding Sites Biological Transport/genetics Cattle Chromosomes, Bacterial Escherichia coli/genetics,growth & development,metabolism Genes, Bacterial Genotype Iron/metabolism Kinetics Molecular Sequence Data Open Reading Frames Phenotype Plasmids Restriction Mapping Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid
Chemicals
Iron Adenosine Triphosphatases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kammler M
Lehrstuhl für Mikrobiologie II, Universität Tübingen, Germany.
Schön C
Hantke K
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32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.19.6212-6219.1993
Published
1993-10-00
Pages
6212-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206716
Subset
IM
Databases
GENBANK
X71063
External Links
PubMed source DOI: 10.1128/jb.175.19.6212-6219.1993 PMC Full Text View journal details
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