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PMID: 8415656 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mice with reduced levels of p53 protein exhibit the testicular giant-cell degenerative syndrome.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 19 ·1993-10-01 ·Pages 9075-9

Rotter V, Schwartz D, Almon E, Goldfinger N, Kapon A, Meshorer A, Donehower LA, Levine AJ

Abstract

Transgenic mice which carry hybrid p53 promoter-chloramphenicol acetyltransferase (CAT) transgenes were found to express CAT enzymatic activity predominantly in the testes. Endogenous levels of p53 mRNA and protein were lower than in the nontransgenic control mice. The various p53 promoter-CAT transgenic mice exhibited in their testes multinucleated giant cells, a degenerative syndrome resulting presumably from the inability of the tetraploid primary spermatocytes to complete meiotic division. The giant-cell degenerative syndrome was also observed in some genetic strains of homozygous p53 null mice. In view of the hypothesis that p53 plays a role in DNA repair mechanisms, it is tempting to speculate that the physiological function of p53 that is specifically expressed in the meiotic pachytene phase of spermatogenesis is to allow adequate time for the DNA reshuffling and repair events which occur at this phase to be properly completed. Primary spermatocytes which have reduced p53 levels are probably impaired with respect to DNA repair, thus leading to the development of genetically defective giant cells that do not mature.

MeSH Terms
Animals Chloramphenicol O-Acetyltransferase/biosynthesis,genetics Gene Expression Genes, p53 Homozygote Humans Male Mice Mice, Transgenic Promoter Regions, Genetic RNA, Messenger/analysis,metabolism Recombinant Fusion Proteins/biosynthesis Restriction Mapping Syndrome Testis/pathology Tumor Suppressor Protein p53/biosynthesis
Chemicals
RNA, Messenger Recombinant Fusion Proteins Tumor Suppressor Protein p53 Chloramphenicol O-Acetyltransferase
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Rotter V
Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Schwartz D
Almon E
Goldfinger N
Kapon A
Meshorer A
Donehower L A
Levine A J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.19.9075
Published
1993-10-01
Pages
9075-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC47504
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.90.19.9075 PMC Full Text View journal details
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