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PMID: 8415697 Published · ppublish English Journal Article

Regional expression and chromosomal localization of the delta opiate receptor gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 20 ·1993-10-15 ·Pages 9305-9

Bzdega T, Chin H, Kim H, Jung HH, Kozak CA, Klee WA

Abstract

The delta opiate receptor gene has been cloned from the mouse neuroblastoma-rat glioma hybrid cell NG108-15. The clone that we isolated is apparently identical to that reported by Evans et al. [Evans, C. J., Keith, D. E., Jr., Morrison, H., Magendzo, K. & Edwards, R. H. (1992) Science 258, 1952-1955] and essentially identical with that of Kieffer et al. [Kieffer, B. L., Befort, K., Gaveriaux-Ruff, C. & Hirth, C. G. (1992) Proc. Natl. Acad. Sci. USA 89, 12048-12052]. We have found full-length transcripts of the gene in mouse brain but in no other tissues examined. Within the brain the gene is expressed at low levels in many regions but transcripts are found in particularly large amounts in the anterior pituitary and pineal glands. Since these tissues are located outside the blood-brain barrier, opioid peptides easily can reach receptors in these areas from the blood. The gene, which is present as a single copy, has been mapped to the distal region of mouse Chromosome 4.

Related Genes
Nbor
MeSH Terms
Amino Acid Sequence Animals Base Sequence Brain/metabolism Cloning, Molecular DNA, Complementary/genetics Gene Expression Genes Genetic Linkage In Situ Hybridization Mice Molecular Sequence Data Oligonucleotide Probes RNA, Messenger/genetics Rats Receptors, Opioid, delta/genetics
Chemicals
DNA, Complementary Oligonucleotide Probes RNA, Messenger Receptors, Opioid, delta
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Bzdega T
Laboratory of Molecular Biology, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892.
Chin H
Kim H
Jung H H
Kozak C A
Klee W A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.20.9305
Published
1993-10-15
Pages
9305-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC47556
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.90.20.9305 PMC Full Text View journal details
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