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PMID: 8419305 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Absence of a role for DNA polymerase II in SOS-induced translesion bypass of phi X174.

Journal of bacteriology ·Vol. 175 ·No. 2 ·1993-01-00 ·Pages 561-4

Kow YW, Faundez G, Hays S, Bonner CA, Goodman MF, Wallace SS

Abstract

In order to examine the possible role of Escherichia coli DNA polymerase II in SOS-induced translesion bypass, Weigle reactivation and mutation induction were measured with single-stranded phi X174 transfecting DNA containing individual lesions. No decrease in bypass of thymine glycol or cyclobutane pyrimidine dimers in the absence of DNA polymerase II was observed. Furthermore, DNA polymerase II did not affect bypass of abasic sites when either survival or mutagenesis was the endpoint. Lastly, repair of gapped DNA molecules, intermediates in methyl-directed mismatch repair, was also unaffected by the presence or absence of DNA polymerase II.

Related Genes
dinA polA polB
MeSH Terms
Bacteriophage phi X 174/genetics,radiation effects DNA Damage DNA Polymerase II/genetics,metabolism DNA Repair DNA, Viral/genetics,metabolism Dose-Response Relationship, Radiation Escherichia coli/enzymology,genetics,radiation effects Genes, Bacterial Mutagenesis SOS Response, Genetics Transfection Ultraviolet Rays
Chemicals
DNA, Viral DNA Polymerase II
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kow Y W
Department of Microbiology and Molecular Genetics, University of Vermont, Burlington 05405.
Faundez G
Hays S
Bonner C A
Goodman M F
Wallace S S
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.2.561-564.1993
Published
1993-01-00
Pages
561-4
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC196174
Subset
IM
Grants
NCI NIH HHS · CA33657 · United States
NIGMS NIH HHS · GM21422 · United States
NIGMS NIH HHS · GM42554 · United States
External Links
PubMed source DOI: 10.1128/jb.175.2.561-564.1993 PMC Full Text View journal details
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