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PMID: 8428591 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Clonal deletion of specific thymocytes by an immunoglobulin idiotype.

The EMBO journal ·Vol. 12 ·No. 1 ·1993-01-00 ·Pages 357-63

Bogen B, Dembic Z, Weiss S

Abstract

We have investigated whether immunoglobulin can induce clonal deletion of thymocytes by employing two strains of transgenic mice. One strain is transgenic for an alpha/beta T cell receptor (TCR) which recognizes a processed idiotypic peptide of the lambda 2(315) light chain variable region, bound to the I-Ed class II major histocompatibility complex molecule. The other mouse strain is transgenic for the lambda 2(315) gene. Double transgenic offspring from a TCR-transgenic female mated with a lambda 2(315) transgenic male exhibit a pronounced clonal deletion of CD4+CD8+ thymocytes. Analysis of neonates from the reciprocal (lambda 2(315)-transgenic female x TCR-transgenic male) cross suggests that the deletion in double transgenic offspring most likely is caused by lambda 2(315) produced within the thymus rather than by maternally derived IgG, lambda 2(315). Nevertheless, IgG, lambda 2(315) can cause deletion of CD4+CD8+ thymocytes when injected in large amounts intraperitoneally into either adult or neonatal TCR-transgenic mice. Deletion is evident 48 and 72 h after injection, but by day 7 the thymus has already regained its normal appearance. A serum concentration of several hundred microgram/ml is required for deletion to be observed. Therefore, the heterogeneous idiotypes of serum Ig are probably each of too low concentration to cause thymocyte deletion in normal animals.

MeSH Terms
Animals Antibodies, Monoclonal Clone Cells Crosses, Genetic Enzyme-Linked Immunosorbent Assay Female Flow Cytometry Fluorescent Antibody Technique Immunoglobulin Idiotypes/immunology Immunoglobulin Variable Region/genetics Immunoglobulin lambda-Chains/immunology Lymphocyte Activation Lymphocyte Depletion Male Mice Mice, Transgenic Receptors, Antigen, T-Cell, alpha-beta/genetics T-Lymphocytes/immunology Thymus Gland/immunology
Chemicals
Antibodies, Monoclonal Immunoglobulin Idiotypes Immunoglobulin Variable Region Immunoglobulin lambda-Chains Receptors, Antigen, T-Cell, alpha-beta
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bogen B
Institute of Immunology and Rheumatology, University of Oslo, Norway.
Dembic Z
Weiss S
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-01-00
Pages
357-63
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413213
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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