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PMID: 8432587 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Localization of myosin heavy chain A in the human pathogen Entamoeba histolytica.

Infection and immunity ·Vol. 61 ·No. 3 ·1993-03-00 ·Pages 1048-54

Rahim Z, Raymond-Denise A, Sansonetti P, Guillen N

Abstract

To recognize myosin II in trophozoites of the human pathogen Entamoeba histolytica, a specific antimyosin polyclonal serum was raised against a fusion protein consisting of a 146-amino-acid fragment of the myosin II heavy chain A of E. histolytica (MhcA) fused with beta-galactosidase. The hybrid protein was encoded by a chimera gene formed by a DNA fragment, from the mhcA gene, amplified by polymerase chain reaction and fused with the lacZ gene of Escherichia coli. Polymerase chain reaction-amplified DNA is located within the region encoding the tail domain of myosin. This antibody recognized a 250-kDa protein in extracts of E. histolytica trophozoites. Confocal microscope analysis of antibody-labelled trophozoites indicated that MhcA localizes at the posterior pole of locomoting cells and concentrates within the uroid. These results might indicate that MhcA is involved in movement and in the uroid formation which help amoebas to escape the host immune response. These data are the first evidence indicating that myosin exists in E. histolytica. In addition, two other peptides were found in myosin-enriched extracts of amoebas, indicating that other myosins may be present in this parasite.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Compartmentation Cell Movement Entamoeba histolytica/chemistry,ultrastructure Fluorescent Antibody Technique Molecular Sequence Data Myosins/physiology Oligodeoxyribonucleotides/chemistry Protozoan Proteins/chemistry,metabolism Recombinant Fusion Proteins/immunology Restriction Mapping
Chemicals
Oligodeoxyribonucleotides Protozoan Proteins Recombinant Fusion Proteins Myosins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rahim Z
Unité de Pathogénie Microbienne Moléculaire, U 199 INSERM, Institut Pasteur, Paris, France.
Raymond-Denise A
Sansonetti P
Guillen N
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.61.3.1048-1054.1993
Published
1993-03-00
Pages
1048-54
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC302837
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.61.3.1048-1054.1993 PMC Full Text View journal details
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