Home LiteratureArticle Details
PMID: 8432726 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification and characterization of thrombospondin-4, a new member of the thrombospondin gene family.

The Journal of cell biology ·Vol. 120 ·No. 4 ·1993-02-00 ·Pages 1059-67

Lawler J, Duquette M, Whittaker CA, Adams JC, McHenry K, DeSimone DW

Abstract

A new member of the thrombospondin gene family, designated thrombospondin-4, has been identified in the Xenopus laevis genome. The predicted amino acid sequence indicates that the protein is similar to the other members of this gene family in the structure of the type 3 repeats and the COOH-terminal domain. Thrombospondin-4 contains four type 2 repeats and lacks the type 1 repeats that are found in thrombospondin-1 and 2. The amino-terminal domain of thrombospondin-4 has no significant homology with the other members of the thrombospondin gene family or with other proteins in the database. RNAse protection analysis establishes that the initial expression of Xenopus thrombospondin-4 is observed during neurulation. Levels of mRNA expression increase twofold during tailbud stages but decrease by the feeding tadpole stage. The size of the thrombospondin-4 message is 3.3 Kb and 3.4 Kb in the frog and human, respectively. Northern blot analysis of human tissues reveals high levels of thrombospondin-4 expression in heart and skeletal muscle, low levels in brain, lung and pancreas and undetectable levels in the placenta, liver and kidney. These data establish the existence of a new member of the thrombospondin gene family that may participate in the genesis and function of cardiac and skeletal muscle.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular Gene Expression Genes Humans Molecular Sequence Data Multigene Family Oligodeoxyribonucleotides/chemistry Platelet Membrane Glycoproteins/genetics Polymerase Chain Reaction RNA, Messenger/genetics Repetitive Sequences, Nucleic Acid Restriction Mapping Sequence Alignment Thrombospondins Xenopus laevis/embryology,genetics
Chemicals
Oligodeoxyribonucleotides Platelet Membrane Glycoproteins RNA, Messenger Thrombospondins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Lawler J
Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts.
Duquette M
Whittaker C A
Adams J C
McHenry K
DeSimone D W
References (53)
53 references, click to expand
  1. Thrombospondin and a 140 kd fragment promote adhesion and neurite outgrowth from embryonic central and peripheral neurons and from PC12 cells.
    Neuron. 1991 Aug;7(2):231-7 PMID: 1873028
  2. Cell attachment to thrombospondin: the role of ARG-GLY-ASP, calcium, and integrin receptors.
    J Cell Biol. 1988 Dec;107(6 Pt 1):2351-61 PMID: 2848850
  3. Adhesion to thrombospondin by human embryonic fibroblasts is mediated by multiple receptors and includes a role for glycoprotein 88 (CD36).
    Exp Cell Res. 1992 Jan;198(1):85-92 PMID: 1370062
  4. Cell attachment activity of the carboxyl-terminal domain of human thrombospondin expressed in Escherichia coli.
    J Biol Chem. 1991 Dec 25;266(36):24257-9 PMID: 1761530
  5. Identification and characterization of alternatively spliced fibronectin mRNAs expressed in early Xenopus embryos.
    Dev Biol. 1992 Feb;149(2):357-69 PMID: 1730390
  6. Biological activities of peptides and peptide analogues derived from common sequences present in thrombospondin, properdin, and malarial proteins.
    J Cell Biol. 1992 Jan;116(1):209-17 PMID: 1730745
  7. Characterization of the murine thrombospondin gene.
    Genomics. 1991 Nov;11(3):587-600 PMID: 1774063
  8. Expression and mutagenesis of thrombospondin.
    Biochemistry. 1992 Feb 4;31(4):1173-80 PMID: 1734965
  9. Thrombospondin sequence motif (CSVTCG) is responsible for CD36 binding.
    Biochem Biophys Res Commun. 1992 Feb 14;182(3):1208-17 PMID: 1371676
  10. Heparin- and sulfatide-binding peptides from the type I repeats of human thrombospondin promote melanoma cell adhesion.
    Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):3040-4 PMID: 1557410
  11. Thrombospondin 3 (Thbs3), a new member of the thrombospondin gene family.
    J Biol Chem. 1992 Jun 15;267(17):12192-6 PMID: 1601886
  12. Colocalization of thrombospondin and syndecan during murine development.
    Dev Dyn. 1992 Apr;193(4):346-58 PMID: 1380845
  13. UNC-5, a transmembrane protein with immunoglobulin and thrombospondin type 1 domains, guides cell and pioneer axon migrations in C. elegans.
    Cell. 1992 Oct 16;71(2):289-99 PMID: 1384987
  14. Albumin phylogeny for clawed frogs (Xenopus).
    Science. 1977 Feb 25;195(4280):785-7 PMID: 65013
  15. Unique distribution of the extracellular matrix component thrombospondin in the developing mouse embryo.
    J Cell Biol. 1988 Dec;107(6 Pt 2):2737-48 PMID: 3204123
  16. Heparan sulfate-mediated binding of epithelial cell surface proteoglycan to thrombospondin.
    J Biol Chem. 1989 Feb 15;264(5):2885-9 PMID: 2521631
  17. The mRNA encoding elongation factor 1-alpha (EF-1 alpha) is a major transcript at the midblastula transition in Xenopus.
    Dev Biol. 1989 May;133(1):93-100 PMID: 2707491
  18. The GPIIB-IIIa-like complex may function as a human melanoma cell adhesion receptor for thrombospondin.
    Exp Cell Res. 1989 Jun;182(2):473-81 PMID: 2470606
  19. An integrin receptor on normal and thrombasthenic platelets that binds thrombospondin.
    Blood. 1989 Nov 1;74(6):2022-7 PMID: 2478219
  20. The interaction of thrombospondin with platelet glycoprotein GPIIb-IIIa.
    J Biol Chem. 1989 Dec 15;264(35):21322-6 PMID: 2592377
  21. Structure and chromosomal localization of the human thrombospondin gene.
    Genomics. 1990 Apr;6(4):685-91 PMID: 2341158
  22. Characterization of the mouse thrombospondin gene and evaluation of the role of the first intron in human gene expression.
    J Biol Chem. 1990 Sep 25;265(27):16691-8 PMID: 2398070
  23. Cell-adhesive motif in region II of malarial circumsporozoite protein.
    Science. 1990 Sep 28;249(4976):1574-7 PMID: 2120774
  24. Structure and expression of the human polymorphic epithelial mucin gene: an expressed VNTR unit.
    Biochem Biophys Res Commun. 1990 Dec 31;173(3):1019-29 PMID: 2268309
  25. Cellular attachment to thrombospondin. Cooperative interactions between receptor systems.
    J Biol Chem. 1991 Jan 25;266(3):1740-5 PMID: 1703153
  26. The properdin-like type I repeats of human thrombospondin contain a cell attachment site.
    J Cell Biol. 1991 Mar;112(5):1031-40 PMID: 1999454
  27. Vitronectin and thrombospondin promote retinal neurite outgrowth: developmental regulation and role of integrins.
    Neuron. 1991 Mar;6(3):345-58 PMID: 1705807
  28. Thrombospondin-induced adhesion of human platelets.
    J Clin Invest. 1991 Apr;87(4):1387-94 PMID: 2010551
  29. Cloning and sequencing of chicken thrombospondin.
    J Biol Chem. 1991 May 5;266(13):8039-43 PMID: 2022631
  30. Key residues involved in calcium-binding motifs in EGF-like domains.
    Nature. 1991 May 9;351(6322):164-7 PMID: 2030732
  31. A second, expressed thrombospondin gene (Thbs2) exists in the mouse genome.
    J Biol Chem. 1991 Jul 15;266(20):12821-4 PMID: 1712771
  32. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  33. Synthesis and processing of asparagine-linked oligosaccharides.
    Annu Rev Biochem. 1981;50:555-83 PMID: 7023366
  34. A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
    Cell. 1982 Oct;30(3):675-86 PMID: 6183003
  35. Cooperative binding of calcium to thrombospondin. The effect of calcium on the circular dichroism and limited tryptic digestion of thrombospondin.
    J Biol Chem. 1983 Oct 25;258(20):12098-101 PMID: 6630182
  36. Antibodies against a 23Kd heparin binding fragment of thrombospondin inhibit platelet aggregation.
    Biochem Biophys Res Commun. 1984 Oct 15;124(1):290-5 PMID: 6497883
  37. The structure of human platelet thrombospondin.
    J Biol Chem. 1985 Mar 25;260(6):3762-72 PMID: 2579080
  38. A monoclonal antibody against human thrombospondin inhibits platelet aggregation.
    Proc Natl Acad Sci U S A. 1985 May;82(10):3472-6 PMID: 2582413
  39. The platelet glycoprotein thrombospondin binds specifically to sulfated glycolipids.
    J Biol Chem. 1985 Aug 5;260(16):9405-11 PMID: 3926767
  40. Mapping of epitopes for monoclonal antibodies against human platelet thrombospondin with electron microscopy and high sensitivity amino acid sequencing.
    J Cell Biol. 1985 Oct;101(4):1434-41 PMID: 2413043
  41. A new method for predicting signal sequence cleavage sites.
    Nucleic Acids Res. 1986 Jun 11;14(11):4683-90 PMID: 3714490
  42. Thrombospondin-induced attachment and spreading of human squamous carcinoma cells.
    Exp Cell Res. 1986 Dec;167(2):376-90 PMID: 3770094
  43. The structure of human thrombospondin, an adhesive glycoprotein with multiple calcium-binding sites and homologies with several different proteins.
    J Cell Biol. 1986 Nov;103(5):1635-48 PMID: 2430973
  44. Platelet thrombospondin mediates attachment and spreading of human melanoma cells.
    J Cell Biol. 1987 Jan;104(1):131-9 PMID: 3793757
  45. Thrombin and chymotrypsin interactions with thrombospondin.
    Ann N Y Acad Sci. 1986;485:273-87 PMID: 3471148
  46. Occurrence of beta-hydroxylated asparagine residues in non-vitamin K-dependent proteins containing epidermal growth factor-like domains.
    Proc Natl Acad Sci U S A. 1987 Nov;84(22):7856-60 PMID: 2825166
  47. Thrombospondin and the adhesive behavior of platelets.
    Semin Thromb Hemost. 1987 Jul;13(3):290-7 PMID: 3686021
  48. beta-Hydroxyaspartic acid or beta-hydroxyasparagine in bovine low density lipoprotein receptor and in bovine thrombomodulin.
    J Biol Chem. 1988 Jan 5;263(1):21-4 PMID: 2826439
  49. Cell attachment and fibrinogen binding properties of platelet and endothelial cell thrombospondin are not affected by structural differences in the 70 and 18 kDa protease-resistant domains.
    FEBS Lett. 1988 Feb 15;228(2):215-8 PMID: 3342880
  50. Xenopus laevis integrins. Structural conservation and evolutionary divergence of integrin beta subunits.
    J Biol Chem. 1988 Apr 15;263(11):5333-40 PMID: 2833505
  51. Thrombospondin-induced adhesion of human keratinocytes.
    J Clin Invest. 1988 May;81(5):1537-44 PMID: 2452837
  52. Beta-hydroxyaspartic acid in the first epidermal growth factor-like domain of protein C. Its role in Ca2+ binding and biological activity.
    J Biol Chem. 1988 Dec 15;263(35):19240-8 PMID: 2461936
  53. A second thrombospondin gene in the mouse is similar in organization to thrombospondin 1 but does not respond to serum.
    Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8636-40 PMID: 1924325
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.120.4.1059
Published
1993-02-00
Pages
1059-67
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2200072
Subset
IM
Grants
NICHD NIH HHS · R01 HD026402 · United States
NICHD NIH HHS · HD26402 · United States
NHLBI NIH HHS · HL28749 · United States
NHLBI NIH HHS · HL42443 · United States
Databases
GENBANK
Z19091
External Links
PubMed source DOI: 10.1083/jcb.120.4.1059 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2