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PMID: 8437569 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Genetic analysis of the bchC and bchA genes of Rhodobacter sphaeroides.

Molecular & general genetics : MGG ·Vol. 236 ·No. 2-3 ·1993-01-00 ·Pages 227-34

McGlynn P, Hunter CN

Abstract

This study has identified by sequence analysis a single gene in the bchC locus of Rhodobacter sphaeroides and three genes, designated bchX, Y and Z, in the bchA locus, which was previously thought to contain only a single gene. All four genes may reside within the same operon and are transcribed in the order bchC-X-Y-Z. Complementation analysis of eight transposon insertion mutants within these genes suggests that bchX, Y and Z are essential for the reduction of 2-devinyl-2-hydroxyethyl chlorophyllide a and that bchC encodes the 2-desacetyl-2-hydroxyethyl bacteriochlorophyllide a dehydrogenase. Similarity between the putative BchX protein and dinitrogenase reductase proteins suggests that BchX may also be a reductase, supplying electrons for reduction of 2-devinyl-2-hydroxyethyl chlorophyllide a.

Related Genes
bchA bchC bchX bchY bchZ
MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Bacteriochlorophylls/biosynthesis Base Sequence Genes, Bacterial/genetics Genetic Complementation Test Molecular Sequence Data Mutagenesis, Insertional Oxidation-Reduction Oxidoreductases/genetics RNA Precursors/genetics Rhodobacter sphaeroides/genetics Sequence Analysis, DNA
Chemicals
Bacterial Proteins Bacteriochlorophylls RNA Precursors bacteriochlorophyll A proteins, Bacteria Oxidoreductases 2-desacetyl-2-hydroxyethyl bacteriochlorophyllide a dehydrogenase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
McGlynn P
Krebs Institute for Biomolecular Research, University of Sheffield, UK.
Hunter C N
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18 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00277117
Published
1993-01-00
Pages
227-34
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00277117
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