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PMID: 1701878 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Localization and structural analysis of the ribosomal RNA operons of Rhodobacter sphaeroides.

Nucleic acids research ·Vol. 18 ·No. 24 ·1990-12-25 ·Pages 7267-77

Dryden SC, Kaplan S

Abstract

We have identified, cloned and sequenced the three ribosomal RNA (rRNA) operons (rrn) present in the facultative photoheterotroph Rhodobacter sphaeroides. DNA sequence analysis has identified the 16S, 23S, and 5S rRNAs, two tRNAs (ile and ala) in the spacer region between the 16S and 23S rRNAs, and an f-met tRNA immediately following the 5S rRNA gene of all three operons. Physical mapping, genetic analysis, and Southern hybridization data indicate that rrnA is contained on a large chromosome and rrnB and rrnC are contained on a second smaller chromosome. These findings are discussed in relation to the origins of diploidy.

Related Genes
rrnA rrnB rrnC
MeSH Terms
Base Sequence Blotting, Southern Cloning, Molecular Genes, Bacterial Molecular Sequence Data Nucleic Acid Conformation Operon RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/genetics RNA, Ribosomal, 5S/genetics Restriction Mapping Rhodobacter sphaeroides/genetics
Chemicals
RNA, Bacterial RNA, Ribosomal, 16S RNA, Ribosomal, 23S RNA, Ribosomal, 5S
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dryden S C
Department of Microbiology, University of Texas Health Science Center, Houston 77225.
Kaplan S
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37 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.24.7267
Published
1990-12-25
Pages
7267-77
Language
English
Region
England
NLM ID
0411011
PMCID
PMC332862
Subset
IM
Grants
NIGMS NIH HHS · GM31667 · United States
Databases
GENBANK
X53853, X53854, X53855
External Links
PubMed source DOI: 10.1093/nar/18.24.7267 PMC Full Text View journal details
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