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PMID: 2509424 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Physical and genetic maps of the genome of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120.

Journal of bacteriology ·Vol. 171 ·No. 11 ·1989-11-00 ·Pages 5940-8

Bancroft I, Wolk CP, Oren EV

Abstract

A restriction map of the chromosome of the cyanobacterium Anabaena sp. strain PCC 7120 was generated by the determination of the order of restriction fragments of the infrequently cleaving restriction endonucleases AvrII, SalI, and PstI. These restriction fragments were resolved by the pulsed homogeneous orthogonal field gel electrophoresis system of pulsed-field gel electrophoresis (I. Bancroft and C. P. Wolk, Nucleic Acids Res. 16:7405-7418, 1988). Other infrequently cutting restriction endonucleases (AhaII, Asp718, AsuII, BanII, BglII, BssHII, FspI, NcoI, NruI, SphI, SplI, SstII, and StuI) were identified that could prove useful for higher-resolution mapping. The chromosome was found to be 6.4 megabases in size and circular. Three apparently circular large plasmids (410, 190, and 110 kilobases) were also identified. A genetic map was constructed by hybridization with gene-specific probes. Genes encoding components of the photosynthetic electron transport chain were not within a single tight cluster.

MeSH Terms
Chromosome Mapping Cloning, Molecular Cyanobacteria/genetics,physiology DNA/genetics,isolation & purification Molecular Weight Nucleic Acid Hybridization Restriction Mapping
Chemicals
DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bancroft I
MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing 48824-1312.
Wolk C P
Oren E V
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.11.5940-5948.1989
Published
1989-11-00
Pages
5940-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210458
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.171.11.5940-5948.1989 PMC Full Text View journal details
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