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PMID: 2900236 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Genes encoding the alpha, gamma, delta, and four F0 subunits of ATP synthase constitute an operon in the cyanobacterium Anabaena sp. strain PCC 7120.

Journal of bacteriology ·Vol. 170 ·No. 8 ·1988-08-00 ·Pages 3448-58

McCarn DF, Whitaker RA, Alam J, Vrba JM, Curtis SE

Abstract

A cluster of genes encoding subunits of ATP synthase of Anabaena sp. strain PCC 7120 was cloned, and the nucleotide sequences of the genes were determined. This cluster, denoted atp1, consists of four F0 genes and three F1 genes encoding the subunits a (atpI), c (atpH), b' (atpG), b (atpF), delta (atpD), alpha (aptA), and gamma (atpC) in that order. Closely linked upstream of the ATP synthase subunit genes is an open reading frame denoted gene 1, which is equivalent to the uncI gene of Escherichia coli. The atp1 gene cluster is at least 10 kilobase pairs distant in the genome from apt2, a cluster of genes encoding the beta (atpB) and epsilon (atpE) subunits of the ATP synthase. This two-clustered ATP synthase gene arrangement is intermediate between those found in chloroplasts and E. coli. A unique feature of the Anabaena atp1 cluster is overlap between the coding regions for atpF and atpD. The atp1 cluster is transcribed as a single 7-kilobase polycistronic mRNA that initiates 140 base pairs upstream of gene 1. The deduced translation products for the Anabaena sp. strain PCC 7120 subunit genes are more similar to chloroplast ATP synthase subunits than to those of E. coli.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Cyanobacteria/enzymology,genetics DNA/genetics Genes Molecular Sequence Data Multigene Family Nucleic Acid Hybridization Operon Proton-Translocating ATPases/genetics RNA, Messenger/genetics Repetitive Sequences, Nucleic Acid Transcription, Genetic
Chemicals
RNA, Messenger DNA Proton-Translocating ATPases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
McCarn D F
Department of Genetics, North Carolina State University, Raleigh 27695-7614.
Whitaker R A
Alam J
Vrba J M
Curtis S E
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.8.3448-3458.1988
Published
1988-08-00
Pages
3448-58
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211314
Subset
IM
Grants
NIGMS NIH HHS · GM32766 · United States
Databases
GENBANK
M21659
External Links
PubMed source DOI: 10.1128/jb.170.8.3448-3458.1988 PMC Full Text View journal details
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