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PMID: 16453616 Published · ppublish English Journal Article

The wheat chloroplast gene for CF(0) subunit I of ATP synthase contains a large intron.

The EMBO journal ·Vol. 4 ·No. 6 ·1985-06-00 ·Pages 1381-8

Bird CR, Koller B, Auffret AD, Huttly AK, Howe CJ, Dyer TA, Gray JC

Abstract

The gene for CF(0) subunit I of ATP synthase has been located in wheat chloroplast DNA, between the genes for CF(0) subunit III and alpha subunit of CF(1). Nucleotide sequencing and analysis of RNA-DNA hybrids indicated that the gene is interrupted by an 823-bp intron which has boundaries similar to those previously described for the introns in protein-coding chloroplast genes of Euglena gracilis. The deduced amino acid sequence of CF(0) subunit I indicates a polypeptide of 183 amino acid residues. However, N-terminal amino acid sequencing of the mature spinach CF(0) subunit I suggests that the protein is synthesised with a N-terminal extension of 17 amino acid residues and is processed to give a protein of mol. wt. 19 001 of 166 amino acids residues. The mature CF(0) subunit I shows similarities in primary and predicted secondary structure to F(0) subunit b of Escherichia coli ATP synthase. A major transcript of 3.3 kb containing sequences from the genes for CF(0) subunit III, subunit I and CF(1) subunit alpha has been observed by RNA-DNA hybridisation.

Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Bird C R
Botany School, University of Cambridge, Cambridge CB2 3EA, UK.
Koller B
Auffret A D
Huttly A K
Howe C J
Dyer T A
Gray J C
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-06-00
Pages
1381-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554355
External Links
PubMed source PMC Full Text View journal details
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