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PMID: 6431398 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Chloroplast gene for Mr 32000 polypeptide of photosystem II in Euglena gracilis is interrupted by four introns with conserved boundary sequences.

Nucleic acids research ·Vol. 12 ·No. 14 ·1984-07-25 ·Pages 5801-12

Karabin GD, Farley M, Hallick RB

Abstract

The gene for the Mr 32000 herbicide binding polypeptide of photosystem II has previously been mapped to the 5 kbp EcoRI fragment Eco I of Euglena gracilis chloroplast DNA. The nucleotide sequence of 3324 bp of Eco I, containing the psbA locus, has been determined. This locus encodes a polypeptide of 345 amino acids which is co-linear with, and has 86% derived amino acid sequence homology to sequences derived from four higher plants chloroplast psbA loci. The Euglena psbA gene contains four introns of size 435, 443, 434, and 617 bp. The four introns have conserved boundary sequences of the type previously described in the Euglena chloroplast gene (rbcL) for the large subunit of ribulose-1,5-bisphosphate carboxylase (Koller et al., Cell 36, 545-553, 1984).

MeSH Terms

Base Sequence Chloroplasts/physiology Codon Euglena gracilis/genetics Genes Genetic Linkage Membrane Proteins/genetics Molecular Weight Operon

Chemicals

Codon Membrane Proteins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Karabin G D

Farley M

Hallick R B

References (23)

23 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/12.14.5801

Published

1984-07-25

Pages

5801-12

Language

English

Region

England

NLM ID

0411011

PMCID

PMC320032

Subset

Grants

NIGMS NIH HHS · GM21351 · United States

Databases

GENBANK

X00735

External Links

PubMed source DOI: 10.1093/nar/12.14.5801 PMC Full Text View journal details

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Chloroplast gene for Mr 32000 polypeptide of photosystem II in Euglena gracilis is interrupted by four introns with conserved boundary sequences.

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