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PMID: 2832390 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Restriction analysis and quantitative estimation of methylated bases of filamentous and unicellular cyanobacterial DNAs.

Journal of bacteriology ·Vol. 170 ·No. 4 ·1988-04-00 ·Pages 1934-9

Padhy RN, Hottat FG, Coene MM, Hoet PP

Abstract

The DNAs of strains of three cyanobacterial genera (Anabaena, Plectonema, and Synechococcus) were found to be partially or fully resistant to many restriction endonucleases. This could be due to the absence of specific sequences or to modifications, rendering given sequences resistant to cleavage. The latter explanation is substantiated by the content of N6-methyladenine and 5-methylcytosine in these genomes, which is high in comparison with that in other bacterial genomes. dcm- and dam-like methylases are present in the three strains (based on the restriction patterns obtained with the appropriate isoschizomeric enzymes). Their contribution to the overall content of methyladenine and methylcytosine in the genomes was calculated. Partial methylation of GATC sequences was observed in Anabaena DNA. In addition, the GATC methylation patterns might not have been random in the three cyanobacterial DNA preparations, as revealed by the appearance of discrete fragments (possibly of plasmid origin) withstanding cleavage by DpnI (which requires the presence of methyladenine in the GATC sequence).

MeSH Terms
5-Methylcytosine Adenine/analogs & derivatives,analysis Base Sequence Chromatography, High Pressure Liquid Cyanobacteria/genetics Cytosine/analogs & derivatives,analysis DNA/analysis,genetics,metabolism DNA Restriction Enzymes/metabolism Electrophoresis, Agar Gel Genes Methylation
Chemicals
5-Methylcytosine Cytosine DNA DNA Restriction Enzymes Adenine 6-methyladenine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Padhy R N
Microbiology and Genetics Unit, University of Louvain Medical School, Brussels, Belgium.
Hottat F G
Coene M M
Hoet P P
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.4.1934-1939.1988
Published
1988-04-00
Pages
1934-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211053
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.170.4.1934-1939.1988 PMC Full Text View journal details
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