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PMID: 3108239 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Molecular cloning and characterization of the recA gene from the cyanobacterium Synechococcus sp. strain PCC 7002.

Journal of bacteriology ·Vol. 169 ·No. 6 ·1987-06-00 ·Pages 2739-47

Murphy RC, Bryant DA, Porter RD, de Marsac NT

Abstract

The recA gene of Synechococcus sp. strain PCC 7002 was detected and cloned from a lambda gtwes genomic library by heterologous hybridization by using a gene-internal fragment of the Escherichia coli recA gene as the probe. The gene encodes a 38-kilodalton polypeptide which is antigenically related to the RecA protein of E. coli. The nucleotide sequence of a portion of the gene was determined. The translation of this region was 55% homologous to the E. coli protein; allowances for conservative amino acid replacements yield a homology value of about 74%. The cyanobacterial recA gene product was proficient in restoring homologous recombination and partial resistance to UV irradiation to recA mutants of E. coli. Heterologous hybridization experiments, in which the Synechococcus sp. strain PCC 7002 recA gene was used as the probe, indicate that a homologous gene is probably present in all cyanobacterial strains.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Cyanobacteria/genetics Escherichia coli/genetics Gene Expression Regulation Genes Molecular Weight Rec A Recombinases/genetics Recombination, Genetic Sequence Homology, Nucleic Acid
Chemicals
Rec A Recombinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Murphy R C
Bryant D A
Porter R D
de Marsac N T
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42 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.6.2739-2747.1987
Published
1987-06-00
Pages
2739-47
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212179
Subset
IM
Grants
NIGMS NIH HHS · GM31625 · United States
Databases
GENBANK
M16491
External Links
PubMed source DOI: 10.1128/jb.169.6.2739-2747.1987 PMC Full Text View journal details
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