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PMID: 2465199 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Chromosomal organization of rRNA operons in Bacillus subtilis.

Genetics ·Vol. 120 ·No. 3 ·1988-11-00 ·Pages 625-35

Jarvis ED, Widom RL, LaFauci G, Setoguchi Y, Richter IR, Rudner R

Abstract

Integrative mapping with vectors containing ribosomal DNA sequences were used to complete the mapping of the 10 rRNA gene sets in the endospore forming bacterium Bacillus subtilis. Southern hybridizations allowed the assignment of nine operons to distinct BclI restriction fragments and their genetic locus identified by transductional crosses. Nine of the ten rRNA gene sets are located between 0 and 70 degrees on the genomic map. In the region surrounding cysA14, two sets of closely spaced tandem clusters are present. The first (rrnJ and rrnW) is located between purA16 and cysA14 closely linked to the latter; the second (rrnI, rrnH and rrnG) previously mapped within this area is located between attSPO2 and glpT6. The operons at or near the origin of replication (rrnO,rrnA and rrnJ,rrnW) represent "hot spots" of plasmid insertion.

MeSH Terms
Bacillus subtilis/genetics Blotting, Southern Crosses, Genetic Genes, Bacterial Genetic Linkage Multigene Family Operon Plasmids RNA, Bacterial/genetics RNA, Ribosomal/genetics Restriction Mapping/methods Transduction, Genetic Transformation, Genetic
Chemicals
RNA, Bacterial RNA, Ribosomal
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Jarvis E D
Department of Biological Sciences, Hunter College, City University of New York 10021.
Widom R L
LaFauci G
Setoguchi Y
Richter I R
Rudner R
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/120.3.625
Published
1988-11-00
Pages
625-35
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1203540
Subset
IM
Grants
NCRR NIH HHS · RR03037-03 · United States
NCRR NIH HHS · RR8176-06 · United States
External Links
PubMed source DOI: 10.1093/genetics/120.3.625 PMC Full Text View journal details
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