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PMID: 3522548 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Synthesis of linear plasmid multimers in Escherichia coli K-12.

Journal of bacteriology ·Vol. 167 ·No. 1 ·1986-07-00 ·Pages 327-35

Cohen A, Clark AJ

Abstract

Linear plasmid multimers were identified in extracts of recB21 recC22 strains containing derivatives of the ColE1-type plasmids pACYC184 and pBR322. A mutation in sbcB increases the proportion of plasmid DNA as linear multimers. A model to explain this is based on proposed roles of RecBC enzyme and SbcB enzyme (DNA exonuclease I) in preventing two types of rolling-circle DNA synthesis. Support for this hypothesis was obtained by derepressing synthesis of an inhibitor of RecBC enzyme and observing a difference in control of linear multimer synthesis and monomer circle replication. Reinitiation of rolling-circle DNA synthesis was proposed to occur by recA+-dependent and recA+-independent recombination events involving linear multimers. The presence of linear plasmid multimers in recB and recC mutants sheds new light on plasmid recombination frequencies in various mutant strains.

MeSH Terms
DNA, Bacterial/biosynthesis DNA, Circular/biosynthesis Escherichia coli/enzymology,genetics,metabolism Escherichia coli Proteins Exodeoxyribonuclease V Exodeoxyribonucleases/metabolism Genes, Bacterial Kinetics Mutation Plasmids Recombination, Genetic
Chemicals
DNA, Bacterial DNA, Circular Escherichia coli Proteins Exodeoxyribonucleases exodeoxyribonuclease I Exodeoxyribonuclease V exodeoxyribonuclease V, E coli
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cohen A
Clark A J
References (70)
70 references, click to expand
  1. Mu-1 directed inhibition of DNA breakdown in Escherichia coli, recA cells.
    Mol Gen Genet. 1978 Aug 4;164(1):109-12 PMID: 360037
  2. Modulation of the action of the recBC enzyme of Escherichia coli K-12 by Ca2+.
    J Biol Chem. 1979 Sep 10;254(17):8646-52 PMID: 157358
  3. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  4. Pedigrees of some mutant strains of Escherichia coli K-12.
    Bacteriol Rev. 1972 Dec;36(4):525-57 PMID: 4568763
  5. Replication of bacteriophage lambda DNA dependent on the function of host and viral genes. I. Interaction of red, gam and rec.
    J Mol Biol. 1973 Apr 5;75(2):185-212 PMID: 4580674
  6. Genetic analysis of the recF pathway to genetic recombination in Escherichia coli K12: isolation and characterization of mutants.
    J Mol Biol. 1973 Oct 25;80(2):327-44 PMID: 4587405
  7. On maturation of the bacteriophage lambda chromosome.
    Mol Gen Genet. 1973 Dec 31;127(4):285-95 PMID: 4594001
  8. Inactivation of the ATP-dependent DNase of Escherichia coli after infection with double-stranded DNA phages.
    J Virol. 1974 Dec;14(6):1611-2 PMID: 4610190
  9. Isolation of exonuclease VIII: the enzyme associated with sbcA indirect suppressor.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3593-7 PMID: 4610579
  10. Assay for type II restriction endonucleases using the Escherichia coli recBC DNase and duplex circular DNA.
    Methods Enzymol. 1980;65(1):26-8 PMID: 6246350
  11. Unwinding and rewinding of DNA by the RecBC enzyme.
    Cell. 1980 Nov;22(2 Pt 2):447-57 PMID: 6256081
  12. Genetic analysis of the RecE pathway of genetic recombination in Escherichia coli K-12.
    J Bacteriol. 1981 Jan;145(1):521-32 PMID: 6257642
  13. The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl-paper.
    Anal Biochem. 1980 Nov 15;109(1):123-9 PMID: 6162401
  14. A rapid boiling method for the preparation of bacterial plasmids.
    Anal Biochem. 1981 Jun;114(1):193-7 PMID: 6269464
  15. recA-independent general genetic recombination of plasmids.
    Nature. 1981 Nov 12;294(5837):184-6 PMID: 7029308
  16. Beta protein of bacteriophage lambda promotes renaturation of DNA.
    J Biol Chem. 1981 Dec 25;256(24):12636-9 PMID: 6273399
  17. Identification of the Escherichia coli recB and recC gene products.
    Nature. 1981 Dec 10;294(5841):578-80 PMID: 6458768
  18. Interplasmidic and intraplasmidic recombination in Escherichia coli K-12.
    Mol Gen Genet. 1981;184(2):200-7 PMID: 7035834
  19. Physical mapping of the srl recA region of Escherichia coli: analysis of Tn10 generated insertions and deletions.
    Mol Gen Genet. 1981;183(3):497-504 PMID: 6278241
  20. Direct participation of lexA protein in repression of colicin E1 synthesis.
    J Bacteriol. 1982 Jun;150(3):1479-81 PMID: 7042697
  21. The SOS regulatory system of Escherichia coli.
    Cell. 1982 May;29(1):11-22 PMID: 7049397
  22. Initiation of bacteriophage lambda DNA replication in vitro with purified lambda replication proteins.
    Proc Natl Acad Sci U S A. 1982 Oct;79(20):6176-80 PMID: 6216478
  23. Maintenance of the bacteriocinogenic plasmid Clo DF13 in Escherichia coli cells. II. Specific recombination functions involved in plasmid maintenance.
    Mol Gen Genet. 1982;188(2):338-44 PMID: 6296634
  24. Genetic analysis of regulation of the RecF pathway of recombination in Escherichia coli K-12.
    J Bacteriol. 1983 Mar;153(3):1471-8 PMID: 6337999
  25. Effects of a recA operator mutation on mutant phenotypes conferred by lexA and recF mutations.
    Mutat Res. 1982 Nov;106(1):11-26 PMID: 6219286
  26. Plasmidic recombination in Escherichia coli K-12: the role of recF gene function.
    Mol Gen Genet. 1983;189(3):471-4 PMID: 6346018
  27. Escherichia coli RecBC pseudorevertants lacking chi recombinational hotspot activity.
    J Bacteriol. 1983 Aug;155(2):664-80 PMID: 6348024
  28. Exonuclease VIII of Escherichia coli. II. Mechanism of action.
    J Biol Chem. 1983 Sep 10;258(17):10418-24 PMID: 6309804
  29. LexA protein is a repressor of the colicin E1 gene.
    J Biol Chem. 1983 Nov 10;258(21):13258-61 PMID: 6355108
  30. Genetic analysis of the recJ gene of Escherichia coli K-12.
    J Bacteriol. 1984 Jan;157(1):190-6 PMID: 6317649
  31. Exonucleases I, III, and V are required for stability of ColE1-related plasmids in Escherichia coli.
    J Bacteriol. 1984 Feb;157(2):661-4 PMID: 6363393
  32. Multimerization of high copy number plasmids causes instability: CoIE1 encodes a determinant essential for plasmid monomerization and stability.
    Cell. 1984 Apr;36(4):1097-103 PMID: 6323019
  33. Effect of supercoiling on the abortive initiation kinetics of the RNA-I promoter of ColE1 plasmid DNA.
    J Biol Chem. 1984 Sep 25;259(18):11184-7 PMID: 6206065
  34. The effect of nonhomologous DNA sequences on interplasmidic recombination.
    Genetics. 1984 Sep;108(1):39-52 PMID: 6090270
  35. Control of ColE1 plasmid replication: the process of binding of RNA I to the primer transcript.
    Cell. 1984 Oct;38(3):861-70 PMID: 6207934
  36. Transduction of multi-copy plasmid pBR322 by bacteriophage Mu.
    Mol Gen Genet. 1984;197(1):169-74 PMID: 6239967
  37. Absence of RNase H allows replication of pBR322 in Escherichia coli mutants lacking DNA polymerase I.
    Proc Natl Acad Sci U S A. 1984 Dec;81(24):7845-9 PMID: 6096862
  38. A new class of Escherichia coli recBC mutants: implications for the role of RecBC enzyme in homologous recombination.
    Proc Natl Acad Sci U S A. 1984 Dec;81(24):7850-4 PMID: 6393130
  39. Control of ColE1 plasmid replication: initial interaction of RNA I and the primer transcript is reversible.
    Cell. 1985 Mar;40(3):527-35 PMID: 2578886
  40. Genes of the RecE and RecF pathways of conjugational recombination in Escherichia coli.
    Cold Spring Harb Symp Quant Biol. 1984;49:453-62 PMID: 6397300
  41. RecF and RecBC function during recombination of nonreplicating, UV-irradiated phage lambda DNA and during other recombination processes.
    Cold Spring Harb Symp Quant Biol. 1984;49:475-83 PMID: 6241554
  42. Roles of RecBC enzyme and chi sites in homologous recombination.
    Cold Spring Harb Symp Quant Biol. 1984;49:485-95 PMID: 6397303
  43. Chi-dependent DNA strand cleavage by RecBC enzyme.
    Cell. 1985 May;41(1):145-51 PMID: 3888404
  44. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  45. Characteristics of some multiply recombination-deficient strains of Escherichia coli.
    J Bacteriol. 1969 Oct;100(1):231-9 PMID: 4898990
  46. Genetic analysis of recombination-deficient mutants of Escherichia coli K-12 carrying rec mutations cotransducible with thyA.
    J Bacteriol. 1969 Nov;100(2):923-34 PMID: 4901366
  47. Biochemical and genetic studies of recombination proficiency in Escherichia coli. II. Rec+ revertants caused by indirect suppression of rec- mutations.
    Proc Natl Acad Sci U S A. 1970 Sep;67(1):128-35 PMID: 4248156
  48. Genetic recombination in Escherichia coli: the role of exonuclease I.
    Proc Natl Acad Sci U S A. 1971 Apr;68(4):824-7 PMID: 4927675
  49. Indirect suppression of recB and recC mutations by exonuclease I deficiency.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1366-70 PMID: 4556461
  50. Toward a metabolic interpretation of genetic recombination of E. coli and its phages.
    Annu Rev Microbiol. 1971;25:437-64 PMID: 4949037
  51. A role for recombination in the production of "free-loader" lambda bacteriophage particles.
    J Mol Biol. 1972 Jul 14;68(1):57-67 PMID: 4559113
  52. Degradation of bacteriophage lambda deoxyribonucleic acid after restriction by Escherichia coli K-12.
    J Bacteriol. 1972 Oct;112(1):161-9 PMID: 4562392
  53. Interaction of the recombination pathways of bacteriophage lambda and host Escherichia coli: effects on lambda recombination.
    J Mol Biol. 1972 Oct 14;70(3):531-7 PMID: 4563260
  54. Interaction of the recombination pathways of bacteriophage lambda and its host Escherichia coli K12: effects on exonuclease V activity.
    J Mol Biol. 1972 Oct 14;70(3):539-48 PMID: 4563261
  55. Genetic analysis of mutations indirectly suppressing recB and recC mutations.
    Genetics. 1972 Oct;72(2):105-15 PMID: 4567284
  56. A defective phage system reveals bacteriophage T4 replication origins that coincide with recombination hot spots.
    Proc Natl Acad Sci U S A. 1985 May;82(10):3345-9 PMID: 3889905
  57. Identification and genetic analysis of sbcC mutations in commonly used recBC sbcB strains of Escherichia coli K-12.
    J Bacteriol. 1985 Nov;164(2):836-44 PMID: 3932331
  58. Hybridization between Escherichia coli and Shigella.
    J Bacteriol. 1957 Oct;74(4):461-76 PMID: 13475269
  59. Cotransduction with thy of a gene required for genetic recombination in Escherichia coli.
    J Bacteriol. 1967 May;93(5):1729-31 PMID: 5337851
  60. Recombination deficient mutants of Escherichia coli K12 that map between thy A and argA.
    Genetics. 1968 Sep;60(1):19-30 PMID: 4884588
  61. Genetic location of certain mutations conferring recombination deficiency in Escherichia coli.
    J Bacteriol. 1969 Jan;97(1):244-9 PMID: 4884815
  62. Recombination and the Escherichia coli K-12 sex factor F.
    J Bacteriol. 1975 Jan;121(1):36-43 PMID: 1090575
  63. The gamma protein specified by bacteriophage gamma. Structure and inhibitory activity for the recBC enzyme of Escherichia coli.
    J Biol Chem. 1975 Sep 25;250(18):7377-87 PMID: 126236
  64. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  65. Amplification in Escherichia coli of enzymes involved in genetic recombination: construction of hybrid ColE1 plasmids carrying the structural gene for exonuclease I.
    Proc Natl Acad Sci U S A. 1976 Oct;73(10):3492-6 PMID: 790387
  66. Use of polylysine for adsorption of nuclei acids and enzymes to electron microscope specimen films.
    Proc Natl Acad Sci U S A. 1977 Jun;74(6):2311-5 PMID: 329278
  67. Protection of parental T4 DNA from a restriction exonuclease by the product of gene 2.
    J Mol Biol. 1977 Nov;116(4):877-81 PMID: 592405
  68. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  69. Suppressibility of recA, recB, and recC mutations by nonsense suppressors.
    J Bacteriol. 1978 May;134(2):590-6 PMID: 350827
  70. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.167.1.327-335.1986
Published
1986-07-00
Pages
327-35
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212879
Subset
IM
Grants
NIAID NIH HHS · AI05371 · United States
External Links
PubMed source DOI: 10.1128/jb.167.1.327-335.1986 PMC Full Text View journal details
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