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PMID: 8483950 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The sizes of the CDR3 hypervariable regions of the murine T-cell receptor beta chains vary as a function of the recombined germ-line segments.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 9 ·1993-05-01 ·Pages 4319-23

Pannetier C, Cochet M, Darche S, Casrouge A, Zöller M, Kourilsky P

Abstract

A method using PCR amplification and primer extension with fluorescent oligonucleotides was developed to analyze T-cell repertoires. The sizes of the hypervariable CDR3-like regions of the murine T-cell antigen receptor beta chains were measured for all possible V beta-J beta combinations. This analysis shows that beta chains are distributed into at least 2000 groups, a value that provides a lower limit to their complexity. The CDR3 sizes appear to be dependent on the J beta and especially the V beta segment used and correlates with amino acid sequence motifs in the corresponding CDR1 region. This feature of T-cell receptors is discussed.

MeSH Terms
Amino Acid Sequence Animals Cloning, Molecular Codon/genetics DNA/genetics,isolation & purification Genetic Variation Haplotypes Major Histocompatibility Complex Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Inbred Strains Molecular Sequence Data Molecular Weight Oligodeoxyribonucleotides Polymerase Chain Reaction/methods Receptors, Antigen, T-Cell, alpha-beta/genetics,isolation & purification Recombination, Genetic Sequence Homology, Amino Acid T-Lymphocytes/immunology Thymus Gland/immunology
Chemicals
Codon Oligodeoxyribonucleotides Receptors, Antigen, T-Cell, alpha-beta DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Pannetier C
Unité de Biologie Moléculaire du Gène, Institut National de la Santé et de la Recherche Medicale, Institut Pasteur.
Cochet M
Darche S
Casrouge A
Zöller M
Kourilsky P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.9.4319
Published
1993-05-01
Pages
4319-23
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC46498
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.90.9.4319 PMC Full Text View journal details
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