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PMID: 1834457 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A special repertoire of alpha:beta T cells in neonatal mice.

The EMBO journal ·Vol. 10 ·No. 12 ·1991-12-00 ·Pages 3647-54

Bogue M, Candéias S, Benoist C, Mathis D

Abstract

According to several functional criteria, the mature thymocytes of neonatal and adult mice are distinctly different. We wondered whether these differences in function might have a structural correlate: do neonates have a distinct repertoire of alpha:beta T cells? In this study, we have exploited the power of polymerase chain reaction technology to generate large numbers of T cell receptor sequences from sorted thymocyte populations from newborn and adult mice. The newborn-derived sequences show very few N nucleotide additions, usually the major source of diversity in T cell receptors. Most interestingly, the paucity of N insertions appears to be exaggerated by selection events that operate during T cell differentiation in the thymus. The significance of these results is largely: (i) that they parallel recent findings on the B cell repertoire in neonates, raising questions about the reactivities specified by such a special repertoire; and (ii) that they suggest a means to 'tag' T cells exported perinatally, allowing one to test the premise that autoreactive T cells derive preferentially from the newborn repertoire.

MeSH Terms
Animals Animals, Newborn Base Sequence Cell Differentiation Flow Cytometry Mice Mice, Inbred Strains Molecular Sequence Data Polymerase Chain Reaction Receptors, Antigen, T-Cell, alpha-beta/genetics T-Lymphocyte Subsets Thymus Gland/cytology Transcription, Genetic
Chemicals
Receptors, Antigen, T-Cell, alpha-beta
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bogue M
Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Strasbourg, France.
Candéias S
Benoist C
Mathis D
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-12-00
Pages
3647-54
Language
English
Region
England
NLM ID
8208664
PMCID
PMC453096
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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