Home LiteratureArticle Details
PMID: 8503883 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Investigation of the subcellular location of the tetrapyrrole-biosynthesis enzyme coproporphyrinogen oxidase in higher plants.

The Biochemical journal ·Vol. 292 ( Pt 2) ·1993-06-01 ·Pages 503-8

Smith AG, Marsh O, Elder GH

Abstract

The subcellular location of two enzymes in the biosynthetic pathway for protoporphyrin IX, coproporphyrinogen (coprogen) oxidase (EC 1.3.3.3) and protoporphyrinogen (protogen) oxidase (EC 1.3.3.4) has been investigated in etiolated pea (Pisum sativum) leaves and spadices of cuckoo-pint (Arum maculatum). Plant tissue homogenized in isotonic buffer was subjected to subcellular fractionation to prepare mitochondria and plastids essentially free of contamination by other cellular organelles, as determined by marker enzymes. Protogen oxidase activity measured fluorimetrically was reproducibly found in both mitochondria and etioplasts. In contrast, coprogen oxidase could be detected only in etioplasts, using either a coupled fluorimetric assay or a sensitive radiochemical method. The implications of these results for the synthesis of mitochondrial haem in plants is discussed.

MeSH Terms
Cell Fractionation Coproporphyrinogen Oxidase/metabolism Fabaceae/enzymology Organelles/enzymology Oxidoreductases/metabolism Oxidoreductases Acting on CH-CH Group Donors Plants, Medicinal Protoporphyrinogen Oxidase Pyrroles/metabolism Tetrapyrroles
Chemicals
Pyrroles Tetrapyrroles Oxidoreductases Oxidoreductases Acting on CH-CH Group Donors Coproporphyrinogen Oxidase Protoporphyrinogen Oxidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Smith A G
Department of Plant Sciences, University of Cambridge, U.K.
Marsh O
Elder G H
References (26)
26 references, click to expand
  1. Kinetic studies on protoporphyrinogen oxidase inhibition by diphenyl ether herbicides.
    Biochem J. 1991 Jul 1;277 ( Pt 1):17-21 PMID: 1854333
  2. Protoporphyrinogen oxidase inhibition by three peroxidizing herbicides: oxadiazon, LS 82-556 and M&B 39279.
    FEBS Lett. 1989 Mar 13;245(1-2):35-8 PMID: 2522396
  3. Protoheme turnover and chlorophyll synthesis in greening barley tissue.
    Plant Physiol. 1975 Mar;55(3):485-90 PMID: 16659107
  4. Role and location of NAD malic enzyme in thermogenic tissues of Araceae.
    Arch Biochem Biophys. 1983 Dec;227(2):511-21 PMID: 6421232
  5. Two isoenzymes each of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase in spinach leaves.
    Arch Biochem Biophys. 1973 Jan;154(1):438-48 PMID: 4144057
  6. Ferrochelatase of spinach chloroplasts.
    Biochem J. 1968 Mar;107(1):113-9 PMID: 5642615
  7. Purification and properties of coproporphyrinogen oxidase from the yeast Saccharomyces cerevisiae.
    Eur J Biochem. 1986 May 2;156(3):579-87 PMID: 3516695
  8. The subcellular loclization and properties of the ferrochelatase of etiolated barley.
    Biochem J. 1976 May 15;156(2):309-14 PMID: 182145
  9. Fluorometric assays for coproporphyrinogen oxidase and protoporphyrinogen oxidase.
    Anal Biochem. 1985 Aug 15;149(1):248-60 PMID: 3907404
  10. Evidence that the coproporphyrinogen oxidase activity of rat liver is situated in the intermembrane space of mitochondria.
    Biochem J. 1978 May 15;172(2):345-7 PMID: 666752
  11. A radiochemical method for the measurement of coproporphyrinogen oxidase and the utilization of substrates other than coproporphyrinogen III by the enzyme from rat liver.
    Biochem J. 1978 Jan 1;169(1):205-14 PMID: 629746
  12. Biosynthesis of protoheme and heme a from glutamate in maize.
    Plant Physiol. 1986 Aug;81(4):965-71 PMID: 16664966
  13. Formation of Mg-Containing Chlorophyll Precursors from Protoporphyrin IX, delta-Aminolevulinic Acid, and Glutamate in Isolated, Photosynthetically Competent, Developing Chloroplasts.
    Plant Physiol. 1984 Apr;74(4):928-33 PMID: 16663535
  14. tRNA(Glu) as a cofactor in delta-aminolevulinate biosynthesis: steps that regulate chlorophyll synthesis.
    Trends Biochem Sci. 1988 Apr;13(4):139-43 PMID: 3075378
  15. Adenine nucleotide levels in the cytosol, chloroplasts, and mitochondria of wheat leaf protoplasts.
    Plant Physiol. 1982 Oct;70(4):971-7 PMID: 16662653
  16. Coproporphyrinogenase in tobacco (Nicotiana tabacum L.).
    Biochem J. 1970 Apr;117(2):215-20 PMID: 4986869
  17. Subcellular localization of two porphyrin-synthesis enzymes in Pisum sativum (pea) and Arum (cuckoo-pint) species.
    Biochem J. 1988 Jan 15;249(2):423-8 PMID: 3277625
  18. The enzymatic defect in variegate prophyria. Studies with human cultured skin fibroblasts.
    N Engl J Med. 1980 Apr 3;302(14):765-9 PMID: 7354807
  19. The inherited enzymatic defect in porphyria variegata.
    Hum Genet. 1981;58(4):425-8 PMID: 7327566
  20. The structural organization of haem synthesis in rat liver mitochondria.
    Biochem J. 1969 Jul;113(3):507-14 PMID: 4308946
  21. Inhibition of plant protoporphyrinogen oxidase by the herbicide acifluorfen-methyl.
    Plant Physiol. 1989 Aug;90(4):1239-42 PMID: 16666913
  22. Orientation of ferrochelatase in bovine liver mitochondria.
    Biochemistry. 1985 Jan 15;24(2):366-70 PMID: 3884041
  23. Oxidation of protoporphyrinogen to protoporphyrin, a step in chlorophyll and haem biosynthesis. Purification and partial characterization of the enzyme from barley organelles.
    Biochem J. 1987 May 15;244(1):219-24 PMID: 3663113
  24. Haem synthesis during cytochrome P-450 induction in higher plants. 5-Aminolaevulinic acid synthesis through a five-carbon pathway in Helianthus tuberosus tuber tissues aged in the dark.
    Biochem J. 1988 Jan 15;249(2):473-80 PMID: 3342026
  25. Protoporphyrinogen oxidase as a molecular target for diphenyl ether herbicides.
    Biochem J. 1989 May 15;260(1):231-5 PMID: 2775186
  26. The mitochondrial localization of coproporphyrinogen III oxidase.
    Biochem J. 1978 Oct 15;176(1):97-102 PMID: 31872
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2920503
Published
1993-06-01
Pages
503-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1134238
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2920503 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2