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PMID: 8524273 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mechanism of differential utilization of the his3 TR and TC TATA elements.

Molecular and cellular biology ·Vol. 15 ·No. 12 ·1995-12-00 ·Pages 7059-66

Iyer V, Struhl K

Abstract

The yeast his3 promoter region contains two TATA elements, TC and TR, that are differentially utilized in constitutive his3 transcription and Gcn4-activated his3 transcription. TR contains the canonical TATAAA sequence, whereas TC is an extended region that lacks a conventional TATA sequence and does not support transcription in vitro. Surprisingly, differential his3 TATA-element utilization does not depend on specific properties of activator proteins but, rather, is determined by the overall level of his3 transcription. At low levels of transcription, the upstream TC is preferentially utilized, even though it is inherently a much weaker TATA element than TR. The TATA elements are utilized equally at intermediate levels, whereas TR is strongly preferred at high levels of transcription. This characteristic behavior can be recreated by replacing TC with moderately functional derivatives of a conventional TATA element, suggesting that TC is a collection of weak TATA elements. Analysis of promoters containing two biochemically defined TATA elements indicates that differential utilization occurs when the upstream TATA element is weaker than the downstream element. In other situations, the upstream TATA element is preferentially utilized in a manner that is independent of the overall level of transcription. Thus, in promoters containing multiple TATA elements, relative utilization not only depends on the quality and arrangement of the TATA elements but can vary with the overall level of transcriptional stimulation. We suggest that differential TATA utilization results from the combination of an intrinsic preference for the upstream element and functional saturation of weak TATA elements at low levels of transcriptional stimulation.

MeSH Terms
Base Sequence Binding Sites Genes, Fungal Molecular Sequence Data Oligodeoxyribonucleotides Promoter Regions, Genetic Restriction Mapping Saccharomyces cerevisiae/genetics,metabolism TATA Box Transcription, Genetic
Chemicals
Oligodeoxyribonucleotides
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Iyer V
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA.
Struhl K
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43 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.15.12.7059
Published
1995-12-00
Pages
7059-66
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC230961
Subset
IM
Grants
NIGMS NIH HHS · GM30186 · United States
External Links
PubMed source DOI: 10.1128/MCB.15.12.7059 PMC Full Text View journal details
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