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PMID: 7729424 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

TBP mutants defective in activated transcription in vivo.

The EMBO journal ·Vol. 14 ·No. 7 ·1995-04-03 ·Pages 1490-7

Arndt KM, Ricupero-Hovasse S, Winston F

Abstract

The TATA box binding protein (TBP) plays a central and essential role in transcription initiation. At TATA box-containing genes transcribed by RNA polymerase II, TBP binds to the promoter and initiates the assembly of a multiprotein preinitiation complex. Several studies have suggested that binding of TBP to the TATA box is an important regulatory step in transcription initiation in vitro. To determine whether TBP is a target of regulatory factors in vivo, we performed a genetic screen in yeast for TBP mutants defective in activated transcription. One class of TBP mutants identified in this screen comprises inositol auxotrophs that are also defective in using galactose as a carbon source. These phenotypes are due to promoter-specific defects in transcription initiation that are governed by the upstream activating sequence (UAS) and apparently not by the sequence of the TATA element. The finding that these TBP mutants are severely impaired in DNA binding in vitro suggests that transcription initiation at certain genes is regulated at the level of TATA box binding by TBP in vivo.

Related Genes
GAL1 GAL10 SPT15
MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Northern DNA Primers DNA-Binding Proteins/genetics,isolation & purification,metabolism Gene Expression Regulation, Fungal Genes, Fungal Genotype Molecular Sequence Data Point Mutation Polymerase Chain Reaction Promoter Regions, Genetic RNA, Fungal/analysis,biosynthesis Saccharomyces cerevisiae/genetics,growth & development,metabolism TATA-Box Binding Protein Transcription Factors/genetics,isolation & purification,metabolism Transcription, Genetic beta-Galactosidase/biosynthesis
Chemicals
DNA Primers DNA-Binding Proteins RNA, Fungal TATA-Box Binding Protein Transcription Factors beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Arndt K M
Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
Ricupero-Hovasse S
Winston F
References (72)
72 references, click to expand
  1. Effects of activation-defective TBP mutations on transcription initiation in yeast.
    Nature. 1994 May 19;369(6477):252-5 PMID: 8183347
  2. A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II.
    Cell. 1994 May 20;77(4):599-608 PMID: 8187178
  3. Stimulation of GAL4 derivative binding to nucleosomal DNA by the yeast SWI/SNF complex.
    Science. 1994 Jul 1;265(5168):53-60 PMID: 8016655
  4. The SNF/SWI family of global transcriptional activators.
    Curr Opin Cell Biol. 1994 Jun;6(3):396-402 PMID: 7917331
  5. Increased recruitment of TATA-binding protein to the promoter by transcriptional activation domains in vivo.
    Science. 1994 Oct 14;266(5183):280-2 PMID: 7939664
  6. Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C.
    Yeast. 1995 Jan;11(1):53-5 PMID: 7762301
  7. Replacement of chromosome segments with altered DNA sequences constructed in vitro.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):4951-5 PMID: 388424
  8. A GAL10-CYC1 hybrid yeast promoter identifies the GAL4 regulatory region as an upstream site.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7410-4 PMID: 6760197
  9. Diverse transcriptional functions of the multisubunit eukaryotic TFIID complex.
    J Biol Chem. 1992 Jan 15;267(2):679-82 PMID: 1730659
  10. The complexities of eukaryotic transcription initiation: regulation of preinitiation complex assembly.
    Trends Biochem Sci. 1991 Nov;16(11):402-8 PMID: 1776168
  11. A bipartite DNA binding domain composed of direct repeats in the TATA box binding factor TFIID.
    Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):2844-8 PMID: 1557391
  12. Activation of RNA polymerase II transcription by the specific DNA-binding protein LSF. Increased rate of binding of the basal promoter factor TFIIB.
    J Biol Chem. 1992 Apr 15;267(11):7845-55 PMID: 1313810
  13. Specific interaction between the nonphosphorylated form of RNA polymerase II and the TATA-binding protein.
    Cell. 1992 May 29;69(5):871-81 PMID: 1591781
  14. A novel transcription factor reveals a functional link between the RNA polymerase II CTD and TFIID.
    Cell. 1992 May 29;69(5):883-94 PMID: 1591782
  15. Nondissociation of GAL4 and GAL80 in vivo after galactose induction.
    Science. 1992 May 29;256(5061):1333-5 PMID: 1598579
  16. The INO2 gene of Saccharomyces cerevisiae encodes a helix-loop-helix protein that is required for activation of phospholipid synthesis.
    Nucleic Acids Res. 1992 Jun 25;20(12):3253 PMID: 1620625
  17. Eukaryotic activators function during multiple steps of preinitiation complex assembly.
    Nature. 1993 Dec 9;366(6455):531-6 PMID: 8255291
  18. Transcription factor (TF) IIB and TFIIA can independently increase the affinity of the TATA-binding protein for DNA.
    J Biol Chem. 1994 Mar 18;269(11):8280-6 PMID: 8132551
  19. An RNA polymerase II holoenzyme responsive to activators.
    Nature. 1994 Mar 31;368(6470):466-9 PMID: 8133894
  20. Drosophila TAFII150: similarity to yeast gene TSM-1 and specific binding to core promoter DNA.
    Science. 1994 May 13;264(5161):933-41 PMID: 8178153
  21. Construction and use of gene fusions to lacZ (beta-galactosidase) that are expressed in yeast.
    Methods Enzymol. 1983;101:167-80 PMID: 6310320
  22. A synthetic HIS4 regulatory element confers general amino acid control on the cytochrome c gene (CYC1) of yeast.
    Proc Natl Acad Sci U S A. 1985 Jan;82(2):498-502 PMID: 2982161
  23. Specific DNA binding of GAL4, a positive regulatory protein of yeast.
    Cell. 1985 Apr;40(4):767-74 PMID: 3886158
  24. Naturally occurring poly(dA-dT) sequences are upstream promoter elements for constitutive transcription in yeast.
    Proc Natl Acad Sci U S A. 1985 Dec;82(24):8419-23 PMID: 3909145
  25. GAL2 codes for a membrane-bound subunit of the galactose permease in Saccharomyces cerevisiae.
    J Bacteriol. 1986 Apr;166(1):313-8 PMID: 3082856
  26. Photofootprinting in vivo detects transcription-dependent changes in yeast TATA boxes.
    Nature. 1987 Jan 8-14;325(7000):173-7 PMID: 3543694
  27. Multiple global regulators control HIS4 transcription in yeast.
    Science. 1987 Aug 21;237(4817):874-80 PMID: 3303332
  28. Vectors for selective expression of cloned DNAs by T7 RNA polymerase.
    Gene. 1987;56(1):125-35 PMID: 3315856
  29. A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli.
    Gene. 1987;57(2-3):267-72 PMID: 3319781
  30. 5-Fluoroorotic acid as a selective agent in yeast molecular genetics.
    Methods Enzymol. 1987;154:164-75 PMID: 3323810
  31. Saturation mutagenesis of a yeast his3 "TATA element": genetic evidence for a specific TATA-binding protein.
    Proc Natl Acad Sci U S A. 1988 Apr;85(8):2691-5 PMID: 3282236
  32. Mechanism of action of a yeast activator: direct effect of GAL4 derivatives on mammalian TFIID-promoter interactions.
    Cell. 1988 Aug 26;54(5):665-9 PMID: 3044608
  33. Transcription factor ATF interacts with the TATA factor to facilitate establishment of a preinitiation complex.
    Cell. 1988 Sep 23;54(7):1033-42 PMID: 3416354
  34. Yeast regulatory gene GAL3: carbon regulation; UASGal elements in common with GAL1, GAL2, GAL7, GAL10, GAL80, and MEL1; encoded protein strikingly similar to yeast and Escherichia coli galactokinases.
    Mol Cell Biol. 1988 Aug;8(8):3439-47 PMID: 3062381
  35. Five intermediate complexes in transcription initiation by RNA polymerase II.
    Cell. 1989 Feb 24;56(4):549-61 PMID: 2917366
  36. SPT3 interacts with TFIID to allow normal transcription in Saccharomyces cerevisiae.
    Genes Dev. 1992 Jul;6(7):1319-31 PMID: 1628834
  37. Simple derivation of TFIID-dependent RNA polymerase II transcription systems from Schizosaccharomyces pombe and other organisms, and factors required for transcriptional activation.
    Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7659-63 PMID: 1502179
  38. Holo-TFIID supports transcriptional stimulation by diverse activators and from a TATA-less promoter.
    Genes Dev. 1992 Oct;6(10):1964-74 PMID: 1398073
  39. A transcriptionally active form of GAL4 is phosphorylated and associated with GAL80.
    Mol Cell Biol. 1992 Nov;12(11):4981-7 PMID: 1406674
  40. Reconstitution of transcription with five purified initiation factors and RNA polymerase II from Saccharomyces cerevisiae.
    J Biol Chem. 1992 Nov 15;267(32):23376-82 PMID: 1331084
  41. Yeast SNF/SWI transcriptional activators and the SPT/SIN chromatin connection.
    Trends Genet. 1992 Nov;8(11):387-91 PMID: 1332230
  42. Molecular cloning and functional analysis of Drosophila TAF110 reveal properties expected of coactivators.
    Cell. 1993 Jan 29;72(2):247-60 PMID: 7678780
  43. Initiation of transcription by RNA polymerase II: a multi-step process.
    Prog Nucleic Acid Res Mol Biol. 1993;44:67-108 PMID: 8434126
  44. Interaction between an acidic activator and transcription factor TFIIB is required for transcriptional activation.
    Nature. 1993 Jun 24;363(6431):741-4 PMID: 8515819
  45. Immunopurification of yeast TATA-binding protein and associated factors. Presence of transcription factor IIIB transcriptional activity.
    J Biol Chem. 1993 Jul 25;268(21):15325-8 PMID: 8340360
  46. Functional interaction of adenovirus E1A with holo-TFIID.
    Genes Dev. 1993 Sep;7(9):1810-23 PMID: 8370528
  47. Crystal structure of a yeast TBP/TATA-box complex.
    Nature. 1993 Oct 7;365(6446):512-20 PMID: 8413604
  48. Co-crystal structure of TBP recognizing the minor groove of a TATA element.
    Nature. 1993 Oct 7;365(6446):520-7 PMID: 8413605
  49. Drosophila TAFII40 interacts with both a VP16 activation domain and the basal transcription factor TFIIB.
    Cell. 1993 Nov 5;75(3):519-30 PMID: 8221891
  50. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
    Genetics. 1989 May;122(1):19-27 PMID: 2659436
  51. SPT15, the gene encoding the yeast TATA binding factor TFIID, is required for normal transcription initiation in vivo.
    Cell. 1989 Sep 22;58(6):1183-91 PMID: 2673545
  52. Functional distinctions between yeast TATA elements.
    Mol Cell Biol. 1989 Dec;9(12):5298-304 PMID: 2685558
  53. The Saccharomyces cerevisiae INO4 gene encodes a small, highly basic protein required for derepression of phospholipid biosynthetic enzymes.
    J Biol Chem. 1990 Mar 15;265(8):4736-45 PMID: 2155238
  54. GAL4 protein: purification, association with GAL80 protein, and conserved domain structure.
    Mol Cell Biol. 1990 Jun;10(6):2916-23 PMID: 2188103
  55. Direct and selective binding of an acidic transcriptional activation domain to the TATA-box factor TFIID.
    Nature. 1990 Jun 28;345(6278):783-6 PMID: 2193231
  56. Tc, an unusual promoter element required for constitutive transcription of the yeast HIS3 gene.
    Mol Cell Biol. 1990 Sep;10(9):4447-55 PMID: 2201891
  57. RNA polymerase II C-terminal repeat influences response to transcriptional enhancer signals.
    Nature. 1990 Oct 4;347(6292):491-4 PMID: 2215664
  58. Mechanism of action of an acidic transcriptional activator in vitro.
    Cell. 1991 Mar 8;64(5):971-81 PMID: 2001592
  59. A functional interaction between the C-terminal domain of RNA polymerase II and the negative regulator SIN1.
    Cell. 1991 Mar 22;64(6):1135-43 PMID: 2004420
  60. Dominant negative mutations in yeast TFIID define a bipartite DNA-binding region.
    Cell. 1991 Apr 19;65(2):349-57 PMID: 2015629
  61. SPT5, an essential gene important for normal transcription in Saccharomyces cerevisiae, encodes an acidic nuclear protein with a carboxy-terminal repeat.
    Mol Cell Biol. 1991 Jun;11(6):3009-19 PMID: 1840633
  62. Yeast histone H4 N-terminal sequence is required for promoter activation in vivo.
    Cell. 1991 Jun 14;65(6):1023-31 PMID: 2044150
  63. RAP1 is required for BAS1/BAS2- and GCN4-dependent transcription of the yeast HIS4 gene.
    Mol Cell Biol. 1991 Jul;11(7):3642-51 PMID: 1904543
  64. Reduced binding of TFIID to transcriptionally compromised mutants of VP16.
    Nature. 1991 Jun 13;351(6327):588-90 PMID: 1646402
  65. Direct interaction between adenovirus E1A protein and the TATA box binding transcription factor IID.
    Proc Natl Acad Sci U S A. 1991 Jun 15;88(12):5124-8 PMID: 1828892
  66. Isolation of coactivators associated with the TATA-binding protein that mediate transcriptional activation.
    Cell. 1991 Aug 9;66(3):563-76 PMID: 1907890
  67. Adenovirus E1A activation domain binds the basic repeat in the TATA box transcription factor.
    Cell. 1991 Oct 18;67(2):365-76 PMID: 1833071
  68. Initiation of eukaryotic messenger RNA synthesis.
    J Biol Chem. 1991 Sep 25;266(27):17721-4 PMID: 1917915
  69. Binding of general transcription factor TFIIB to an acidic activating region.
    Nature. 1991 Oct 10;353(6344):569-71 PMID: 1922364
  70. Random mutagenesis of gene-sized DNA molecules by use of PCR with Taq DNA polymerase.
    Nucleic Acids Res. 1991 Nov 11;19(21):6052 PMID: 1658751
  71. Coactivators for a proline-rich activator purified from the multisubunit human TFIID complex.
    Genes Dev. 1991 Dec;5(12A):2212-24 PMID: 1748279
  72. The Zta trans-activator protein stabilizes TFIID association with promoter DNA by direct protein-protein interaction.
    Genes Dev. 1991 Dec;5(12B):2441-54 PMID: 1661258
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-04-03
Pages
1490-7
Language
English
Region
England
NLM ID
8208664
PMCID
PMC398236
Subset
IM
Grants
NIGMS NIH HHS · GM45720 · United States
External Links
PubMed source PMC Full Text View journal details
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