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PMID: 1557391 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A bipartite DNA binding domain composed of direct repeats in the TATA box binding factor TFIID.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 7 ·1992-04-01 ·Pages 2844-8

Yamamoto T, Horikoshi M, Wang J, Hasegawa S, Weil PA, Roeder RG

Abstract

Point mutations in residues comprising the interrupted direct repeats of TFIID eliminated DNA binding in an electrophoretic mobility shift assay. In contrast, mutations in nonconserved residues within the direct repeat regions or in lysine residues comprising the intervening basic repeat had no effect on DNA binding. However, small spacing changes (addition or deletion of one to three residues) in the basic repeat eliminated DNA binding. These results argue for a bipartite DNA binding domain composed of direct repeats with a strict spacing and orientation. Surprisingly, some direct repeat mutations that inhibited DNA binding failed to show a corresponding inhibition of basal transcription, indicating compensating interactions of TFIID with other general factors. The implications of these and other recent results for TFIID structure, promoter recognition, and interactions with other factors are discussed.

MeSH Terms
Amino Acid Sequence Binding Sites DNA-Binding Proteins/chemistry,metabolism Fungal Proteins/chemistry,metabolism Molecular Sequence Data Mutagenesis, Site-Directed Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid Saccharomyces cerevisiae Structure-Activity Relationship TATA Box Transcription Factor TFIID Transcription Factors/chemistry,metabolism Transcription, Genetic
Chemicals
DNA-Binding Proteins Fungal Proteins Transcription Factor TFIID Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Yamamoto T
Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, NY 10021.
Horikoshi M
Wang J
Hasegawa S
Weil P A
Roeder R G
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.7.2844
Published
1992-04-01
Pages
2844-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC48759
Subset
IM
Grants
NCI NIH HHS · CA42567 · United States
NIDDK NIH HHS · DK42502 · United States
NIGMS NIH HHS · GM45258 · United States
External Links
PubMed source DOI: 10.1073/pnas.89.7.2844 PMC Full Text View journal details
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