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PMID: 8526523 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequence analysis of the Lactococcus lactis temperate bacteriophage BK5-T and demonstration that the phage DNA has cohesive ends.

Applied and environmental microbiology ·Vol. 61 ·No. 11 ·1995-11-00 ·Pages 4089-98

Boyce JD, Davidson BE, Hillier AJ

Abstract

The Lactococcus lactis temperate bacteriophage BK5-T is a type phage in the lactococcal phage classification (A. W. Jarvis, G. F. Fitzgerald, M. Mata, A. Mercenier, H. Neve, I. B. Powell, C. Ronda, M. Saxelin, and M. Teuber, Intervirology 32:2-9, 1991). The nucleotide sequence of 18,935 bp of the genome of BK5-T was determined and analyzed for the presence of open reading frames and other structural features. Thirty-two open reading frames longer than 60 codons were identified, and these appeared to be grouped into at least seven transcriptional units. A search of the nucleotide sequence for restriction sites identified a small number of discrepancies with the previously published physical map of the BK5-T genome (G. Lakshmidevi, B. E. Davidson, and A. J. Hillier, Appl. Environ. Microbiol. 54:1039-1045, 1988). Subsequent analysis of restriction digests of BK5-T DNA which were heated prior to electrophoresis indicated that BK5-T DNA was not terminally redundant as previously reported but contained cohesive ends.

MeSH Terms
Amino Acid Sequence Bacteriophages/classification,genetics Base Sequence Chromosome Mapping DNA Restriction Enzymes DNA, Circular/chemistry,genetics DNA, Viral/chemistry,genetics Genes, Viral Genome, Viral Lactococcus lactis/virology Molecular Sequence Data Open Reading Frames Operon
Chemicals
DNA, Circular DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Boyce J D
Russell Grimwade School of Biochemistry and Molecular Biology, University of Melbourne, Parkville, Victoria, Australia.
Davidson B E
Hillier A J
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.61.11.4089-4098.1995
Published
1995-11-00
Pages
4089-98
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC167716
Subset
IM
Databases
GENBANK
L44593
External Links
PubMed source DOI: 10.1128/aem.61.11.4089-4098.1995 PMC Full Text View journal details
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