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PMID: 1400210 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Branched-chain amino acid biosynthesis genes in Lactococcus lactis subsp. lactis.

Journal of bacteriology ·Vol. 174 ·No. 20 ·1992-10-00 ·Pages 6580-9

Godon JJ, Chopin MC, Ehrlich SD

Abstract

The genes for biosynthesis of the branched-chain amino acids leucine, isoleucine, and valine in Lactococcus lactis subsp. lactis NCDO2118 were characterized by cloning, complementation in Escherichia coli and Bacillus subtilis, and nucleotide sequence analysis. Nine structural genes are clustered on a 12-kb DNA fragment in the order leuABCD ilvDBNCA. Upstream of these genes, the nucleotide sequence suggests the existence of regulation by transcriptional attenuation. Between the leuD and ilvD genes is an unexpected gene, encoding a protein which belongs to the ATP-binding cassette protein superfamily.

Related Genes
ilv leu
MeSH Terms
Amino Acid Sequence Amino Acids, Branched-Chain/biosynthesis,genetics Bacterial Proteins/biosynthesis,genetics Base Sequence Cloning, Molecular Genes, Bacterial/genetics Genetic Complementation Test Lactococcus lactis/genetics,metabolism Molecular Sequence Data Multigene Family/genetics Nucleic Acid Conformation Open Reading Frames Plasmids/genetics RNA, Messenger/genetics
Chemicals
Amino Acids, Branched-Chain Bacterial Proteins RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Godon J J
Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
Chopin M C
Ehrlich S D
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.20.6580-6589.1992
Published
1992-10-00
Pages
6580-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207629
Subset
IM
Databases
GENBANK
M90761, M94060, M94061, M94062, M94629, M94692, X64378, X64379, X64380, X64381
External Links
PubMed source DOI: 10.1128/jb.174.20.6580-6589.1992 PMC Full Text View journal details
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