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PMID: 8531927 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The size heterogeneity of human lysyl oxidase mRNA is due to alternate polyadenylation site and not alternate exon usage.

Molecular biology reports ·Vol. 21 ·No. 2 ·1995-00-00 ·Pages 95-103

Boyd CD, Mariani TJ, Kim Y, Csiszar K

Abstract

We have isolated the entire gene coding for human lysyl oxidase. Coding and untranslated domains of human lysyl oxidase mRNA were found in 7 exons, distributed throughout approximately 14 kb of human genomic DNA. The appearance of exon sequences in lysyl oxidase mRNA in several human tissues was determined using a reverse transcriptase - PCR assay. In contrast to a previous report, this analysis has unambiguously shown that the size heterogeneity of lysyl oxidase mRNA was not due to alternate usage of any of the exons of the lysyl oxidase gene. Moreover, DNA sequence analysis of the entire 3.8 kb 3'-untranslated region (UTR) within exon 7 revealed multiple poly-adenylation sites which were shown to be differentially expressed in human skin fibroblasts. This differential usage of polyadenylation sites within the 3'-UTR explains the appearance of multiple lysyl oxidase mRNAs of different sizes.

MeSH Terms
Adenosine Monophosphate/genetics Base Sequence Exons/genetics Humans Molecular Sequence Data Organ Specificity Protein-Lysine 6-Oxidase/genetics RNA, Messenger/genetics Sequence Analysis
Chemicals
RNA, Messenger Adenosine Monophosphate Protein-Lysine 6-Oxidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Boyd C D
Department of Surgery, UMDNJ-Robert Wood Johnson Medical School New Brunswick, NJ 08903, USA.
Mariani T J
Kim Y
Csiszar K
References (23)
23 references, click to expand
  1. The complete derived amino acid sequence of human lysyl oxidase and assignment of the gene to chromosome 5 (extensive sequence homology with the murine ras recision gene).
    Matrix. 1992 Jun;12(3):242-8 PMID: 1357535
  2. Determinants of messenger RNA stability.
    Cell. 1987 Jan 16;48(1):5-6 PMID: 2431794
  3. Human placental lysyl oxidase. Purification, partial characterization, and preparation of two specific antisera to the enzyme.
    J Biol Chem. 1984 Jun 10;259(11):6996-7002 PMID: 6144680
  4. Assessment of lysyl oxidase variants by urea gel electrophoresis: evidence against disulfide isomers as bases of the enzyme heterogeneity.
    Anal Biochem. 1985 Sep;149(2):430-7 PMID: 2866732
  5. Structure of the mouse lysyl oxidase gene.
    Genomics. 1993 May;16(2):395-400 PMID: 8100214
  6. Post-translational glycosylation and proteolytic processing of a lysyl oxidase precursor.
    J Biol Chem. 1992 Apr 25;267(12):8666-71 PMID: 1349020
  7. Partial characterization of lysyl oxidase from several human tissues.
    Biochem J. 1985 Sep 15;230(3):639-43 PMID: 2865951
  8. Comparison of lysyl oxidase from bovine lung and aorta.
    Connect Tissue Res. 1986;15(3):173-85 PMID: 2875849
  9. Molecular cloning of human lysyl oxidase and assignment of the gene to chromosome 5q23.3-31.2.
    Genomics. 1991 Nov;11(3):508-16 PMID: 1685472
  10. Properties and function of lysyl oxidase.
    Am J Respir Cell Mol Biol. 1991 Sep;5(3):206-10 PMID: 1680355
  11. Sequences capable of restoring poly(A) site function define two distinct downstream elements.
    EMBO J. 1986 Nov;5(11):2907-13 PMID: 3024967
  12. Rat tropoelastin is synthesized from a 3.5-kilobase mRNA.
    J Biol Chem. 1988 Sep 25;263(27):13504-7 PMID: 2971041
  13. Are U4 small nuclear ribonucleoproteins involved in polyadenylation?
    Nature. 1984 May 10-16;309(5964):179-82 PMID: 6325940
  14. A restriction fragment length polymorphism results in a nonconservative amino acid substitution encoded within the first exon of the human lysyl oxidase gene.
    Genomics. 1993 May;16(2):401-6 PMID: 8100215
  15. Cloning of rat aorta lysyl oxidase cDNA: complete codons and predicted amino acid sequence.
    Biochemistry. 1990 May 22;29(20):4863-70 PMID: 1973052
  16. Purification and properties of four species of lysyl oxidase from bovine aorta.
    Biochem J. 1979 Jan 1;177(1):203-14 PMID: 34386
  17. Purification of lysyl oxidase from piglet skin by selective interaction with Sephacryl S-200.
    Biochem J. 1990 Mar 15;266(3):917-9 PMID: 1970238
  18. A uridylate tract mediates efficient heterogeneous nuclear ribonucleoprotein C protein-RNA cross-linking and functionally substitutes for the downstream element of the polyadenylation signal.
    Mol Cell Biol. 1990 Dec;10(12):6397-407 PMID: 1701018
  19. Evidence for structural similarities in the multiple forms of aortic and cartilage lysyl oxidase and a catalytically quiescent aortic protein.
    J Biol Chem. 1982 Nov 25;257(22):13520-6 PMID: 6128336
  20. Alternate exon usage is a commonly used mechanism for increasing coding diversity within genes coding for extracellular matrix proteins.
    Matrix. 1993 Nov;13(6):457-69 PMID: 8309425
  21. Induction of lung lysyl oxidase activity and lysyl oxidase protein by exposure of rats to cadmium chloride: properties of the induced enzyme.
    Connect Tissue Res. 1991;25(3-4):197-208 PMID: 1676359
  22. Characterization of the human lysyl oxidase gene locus.
    J Biol Chem. 1992 Jul 15;267(20):14382-7 PMID: 1352776
  23. Structural and catalytic properties of copper in lysyl oxidase.
    J Biol Chem. 1990 Nov 5;265(31):19022-7 PMID: 1977746
Article Info
Journal
Molecular biology reports
Abbr.
Mol Biol Rep
ISSN
0301-4851
DOI
10.1007/BF00986499
Published
1995-00-00
Pages
95-103
Language
English
Region
Netherlands
NLM ID
0403234
Subset
IM
Grants
NHLBI NIH HHS · HL37488 · United States
NHLBI NIH HHS · HL39869 · United States
NHLBI NIH HHS · HL42798 · United States
Databases
GENBANK
U22384
External Links
PubMed source DOI: 10.1007/BF00986499 View journal details
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