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PMID: 8550484 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation of Vibrio harveyi acyl carrier protein and the fabG, acpP, and fabF genes involved in fatty acid biosynthesis.

Journal of bacteriology ·Vol. 178 ·No. 2 ·1996-01-00 ·Pages 571-3

Shen Z, Byers DM

Abstract

We report the isolation of Vibrio harveyi acyl carrier protein (ACP) and cloning of a 3,973-bp region containing the fabG (encoding 3-ketoacyl-ACP reductase, 25.5 kDa), acpP (encoding ACP, 8.7 kDa), fabF (encoding 3-ketoacyl-ACP synthase II, 43.1 kDa), and pabC (encoding aminodeoxychorismate lyase, 29.9 kDa) genes. Predicted amino acid sequences were, respectively, 78, 86, 76, and 35% identical to those of the corresponding Escherichia coli proteins. Five of the 11 sequence differences between V. harveyi and E. coli ACP were nonconservative amino acid differences concentrated in a loop region between helices I and II.

MeSH Terms
3-Oxoacyl-(Acyl-Carrier-Protein) Reductase 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase/genetics Acyl Carrier Protein/chemistry,genetics,isolation & purification Alcohol Oxidoreductases/genetics Amino Acid Sequence Base Sequence Cloning, Molecular Fatty Acids/biosynthesis Genes, Bacterial/genetics Molecular Sequence Data Molecular Weight Protein Structure, Tertiary Sequence Analysis Sequence Analysis, DNA Sequence Homology, Amino Acid Vibrio/chemistry,enzymology,genetics
Chemicals
Acyl Carrier Protein Fatty Acids Alcohol Oxidoreductases 3-Oxoacyl-(Acyl-Carrier-Protein) Reductase 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Shen Z
Department of Pediatrics, Atlantic Resarch Centre, Dalhousie University, Halifax, Nova Scotia, Canada.
Byers D M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.178.2.571-573.1996
Published
1996-01-00
Pages
571-3
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177696
Subset
IM
Databases
GENBANK
U39441
External Links
PubMed source DOI: 10.1128/jb.178.2.571-573.1996 PMC Full Text View journal details
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