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PMID: 1961745 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The plastid genome of Cryptomonas phi encodes an hsp70-like protein, a histone-like protein, and an acyl carrier protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 23 ·1991-12-01 ·Pages 10783-7

Wang SL, Liu XQ

Abstract

The plastid genome of Cryptomonas phi, a cryptomonad alga, contains three genes that have not previously been found in any organellar genome. Each of these genes encodes a functional class of organellar gene product not previously reported. The first gene, dnaK, encodes a polypeptide of the hsp70 heat shock protein family. The predicted amino acid sequence of the DnaK protein is 54% identical to that of the Escherichia coli hsp70 protein (DnaK), 50-53% identical to that of two nucleus-encoded mitochondrial hsp70 proteins, and 43-46% identical to that of several eukaryotic cytoplasmic members of the hsp70 protein family. The second gene, hlpA, encodes a polypeptide resembling bacterial histone-like proteins. The predicted amino acid sequence of the HlpA protein is 25-53% identical to that of several bacterial histone-like proteins, and the identity increases to 39-76% over a conserved region corresponding to the long arm that binds DNA. The third gene, acpA, encodes an acyl carrier protein, which is a key cofactor in the synthesis and metabolism of fatty acids. Its predicted amino acid sequence is 36-59% identical to that of eubacterial and plant chloroplast (nucleus-encoded) acyl carrier proteins.

MeSH Terms
Acyl Carrier Protein/genetics Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Cloning, Molecular DNA/genetics,isolation & purification DNA-Binding Proteins/genetics Escherichia coli/genetics Eukaryota/genetics Genome Heat-Shock Proteins/genetics Histones/genetics Molecular Sequence Data Organelles/physiology Plants/genetics Plasmids Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
Acyl Carrier Protein Bacterial Proteins DNA-Binding Proteins Heat-Shock Proteins Histones histone-like protein HU, bacteria DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wang S L
Canadian Institute for Advanced Research, Department of Biochemistry, Dalhousie University, Halifax, NS.
Liu X Q
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.23.10783
Published
1991-12-01
Pages
10783-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53015
Subset
IM
Databases
GENBANK
M76547, M80723, M80724, M81660, M81661, M81662, M81664, M81665, M81666, S68027
External Links
PubMed source DOI: 10.1073/pnas.88.23.10783 PMC Full Text View journal details
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