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PMID: 8557753 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A role for phosphatidylinositol 3-kinase in the regulation of beta 1 integrin activity by the CD2 antigen.

The Journal of cell biology ·Vol. 131 ·No. 6 Pt 2 ·1995-12-00 ·Pages 1867-80

Shimizu Y, Mobley JL, Finkelstein LD, Chan AS

Abstract

The rapid and reversible upregulation of the functional activity of integrin receptors on T lymphocytes is a vital step in the adhesive interactions that occur during successful T cell recognition of foreign antigen and transendothelial migration. Although the ligation of several different cell surface receptors, including the antigen-specific CD3/T cell receptor complex, the CD2, CD7, and CD28 antigens, as well as several chemokine receptors, has been shown to rapidly upregulate integrin function, the intracellular signaling events that initiate this increase in adhesion remain poorly defined. In this study, we have used DNA-mediated gene transfer to explore the role of phosphatidylinositol 3-kinase (PI 3-K) in the upregulation of beta 1 integrin functional activity mediated by the CD2 antigen. CD2 was expressed in the myelomonocytic cell line HL60, which expresses beta 1 integrins that mediate adhesion to fibronectin and VCAM-1 in an activation-dependent manner. Antibody stimulation of CD2 expressed on HL60 transfectants resulted within minutes in increased beta 1-mediated adhesion to fibronectin and VCAM-1 at levels comparable to that obtained upon stimulation with the phorbol ester PMA. A role for PI 3-K in CD2-mediated increases in beta 1 integrin function is suggested by: (a) the ability of the PI 3-K inhibitor wortmannin to completely inhibit CD2-induced increases in beta 1 integrin activity; (b) the association of PI 3-K with CD2; and (c) induced PI 3-K activity upon CD2 stimulation. The mode of association of PI 3-K with CD2 is not mediated by tyrosine phosphorylation-dependent binding of PI 3-K via SH2 domains, since: (a) PI 3-K is associated with CD2 in unstimulated cells; (b) CD2 stimulation fails to increase the amount of associated PI 3-K; and (c) the CD2 cytoplasmic domain lacks tyrosine residues. A role for both protein kinase C and cytoskeletal rearrangements in CD2 regulation of integrin activity is also suggested, since a PKC inhibitor partially inhibits CD2-induced increases in beta 1 integrin function, and CD2 stimulation increases F-actin content in a wortmannin-sensitive manner. Analysis of human peripheral T cells indicated that CD2 stimulation also results in PI 3-K-dependent upregulation of beta 1 integrin activity. Thus, these results demonstrate that CD2 can function as an adhesion regulator in the absence of expression of the CD3/T cell receptor complex; and directly implicate PI 3-K as a critical intracellular mediator involved in the regulation of beta 1 integrin functional activity by the CD2 antigen.

MeSH Terms
Actins/metabolism Androstadienes/pharmacology CD2 Antigens/metabolism Cell Adhesion/physiology Enzyme Inhibitors/pharmacology Gene Expression/physiology HL-60 Cells/enzymology Humans Integrin beta1/genetics,metabolism Leukemia, Promyelocytic, Acute Phosphatidylinositol 3-Kinases Phosphotransferases (Alcohol Group Acceptor)/antagonists & inhibitors,physiology Protein Kinase C/antagonists & inhibitors T-Lymphocytes/enzymology Transfection Up-Regulation/physiology Wortmannin
Chemicals
Actins Androstadienes CD2 Antigens Enzyme Inhibitors Integrin beta1 Phosphatidylinositol 3-Kinases Phosphotransferases (Alcohol Group Acceptor) Protein Kinase C Wortmannin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Shimizu Y
Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis 55455, USA.
Mobley J L
Finkelstein L D
Chan A S
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.131.6.1867
Published
1995-12-00
Pages
1867-80
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120662
Subset
IM
Grants
NIAID NIH HHS · AI31126 · United States
NIAID NIH HHS · AI38474 · United States
External Links
PubMed source DOI: 10.1083/jcb.131.6.1867 PMC Full Text View journal details
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