Home LiteratureArticle Details
PMID: 8599952 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Recombination associated with replication of malarial mitochondrial DNA.

The EMBO journal ·Vol. 15 ·No. 3 ·1996-02-01 ·Pages 684-93

Preiser PR, Wilson RJ, Moore PW, McCready S, Hajibagheri MA, Blight KJ, Strath M, Williamson DH

Abstract

Mitochondrial DNA of the malarial parasite Plasmodium falciparum comprises approximately 20 copies per cell of a 6 kb genome, arranged mainly as polydisperse linear concatemers. In synchronous blood cultures, initiation of mtDNA replication coincides with the start of the 4-5 doublings in nuclear DNA that mark the reproductive phase of the erythrocytic cycle. We show that mtDNA replication coincides with a recombination process reminiscent of the replication mechanism used by certain bacteriophages and plasmids. The few circular forms of mtDNA which are also present do not replicate by a theta mechanism, but are themselves the product of recombination, and we propose they undergo rolling circle activity to generate the linear concatemers.

MeSH Terms
Animals DNA Replication DNA, Circular/biosynthesis,genetics,isolation & purification DNA, Mitochondrial/biosynthesis,genetics,isolation & purification DNA, Protozoan/biosynthesis,genetics,isolation & purification Electrophoresis, Gel, Two-Dimensional Kinetics Microscopy, Electron Multigene Family Plasmodium falciparum/genetics,growth & development,metabolism Recombination, Genetic
Chemicals
DNA, Circular DNA, Mitochondrial DNA, Protozoan
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Preiser P R
The National Institute for Medical Research, London, UK.
Wilson R J
Moore P W
McCready S
Hajibagheri M A
Blight K J
Strath M
Williamson D H
References (39)
39 references, click to expand
  1. Molecular cloning and partial sequence of a 5.8 kilobase pair repetitive DNA from Plasmodium falciparum.
    Mol Biochem Parasitol. 1988 Sep;30(3):289-90 PMID: 3054536
  2. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.
    J Mol Biol. 1962 Jun;4:430-43 PMID: 14498379
  3. Sequence identification of cytochrome b in Plasmodium gallinaceum.
    Mol Cell Biol. 1989 Sep;9(9):3614-20 PMID: 2779560
  4. Characterization of a conserved extrachromosomal element isolated from the avian malarial parasite Plasmodium gallinaceum.
    Mol Cell Biol. 1989 Sep;9(9):3621-9 PMID: 2779561
  5. Rolling circle replication of DNA in yeast mitochondria.
    EMBO J. 1991 Dec;10(12):3923-9 PMID: 1935911
  6. Recombination-dependent concatemeric plasmid replication.
    Microbiol Rev. 1991 Dec;55(4):675-83 PMID: 1779931
  7. Homologies between the contiguous and fragmented rRNAs of the two Plasmodium falciparum extrachromosomal DNAs are limited to core sequences.
    Nucleic Acids Res. 1992 Feb 25;20(4):879-87 PMID: 1542578
  8. The 6-kb element of Plasmodium falciparum encodes mitochondrial cytochrome genes.
    Mol Biochem Parasitol. 1992 May;52(1):145-8 PMID: 1320735
  9. Unusual stability of recombination intermediates made by Escherichia coli RecA protein.
    EMBO J. 1992 Jul;11(7):2685-93 PMID: 1628627
  10. In vivo conformation of mitochondrial DNA in fungi and zoosporic moulds.
    Curr Genet. 1992 Oct;22(4):341-4 PMID: 1394519
  11. The endosymbiont hypothesis revisited.
    Int Rev Cytol. 1992;141:233-357 PMID: 1452433
  12. Linear mitochondrial DNAs of yeasts: frequency of occurrence and general features.
    Mol Cell Biol. 1993 Apr;13(4):2309-14 PMID: 8455612
  13. Microtubular organization visualized by immunofluorescence microscopy during erythrocytic schizogony in Plasmodium falciparum and investigation of post-translational modifications of parasite tubulin.
    Parasitology. 1993 Apr;106 ( Pt 3):223-32 PMID: 8488059
  14. Rolling circle-replicating plasmids from gram-positive and gram-negative bacteria: a wall falls.
    Mol Microbiol. 1993 May;8(5):789-96 PMID: 8355606
  15. Reaching for the ring: the study of mitochondrial genome structure.
    Curr Genet. 1993 Oct;24(4):279-90 PMID: 8252636
  16. A 7.1 kb linear DNA molecule of Theileria parva has scrambled rDNA sequences and open reading frames for mitochondrially encoded proteins.
    EMBO J. 1994 Feb 15;13(4):898-905 PMID: 8112303
  17. DNA replication triggered by double-stranded breaks in E. coli: dependence on homologous recombination functions.
    Cell. 1994 Sep 23;78(6):1051-61 PMID: 7923355
  18. Analysis of Schizosaccharomyces pombe mitochondrial DNA replication by two dimensional gel electrophoresis.
    Chromosoma. 1994 Jun;103(3):162-70 PMID: 7924618
  19. Electron-microscopic visualization of deletion mutations.
    Proc Natl Acad Sci U S A. 1968 May;60(1):243-50 PMID: 5241526
  20. Origin of concatemeric T7 DNA.
    Nat New Biol. 1972 Oct 18;239(94):197-201 PMID: 4507727
  21. Synthesis and turnover of Euglena gracilis mitochondrial DNA.
    J Mol Biol. 1974 Jan 5;82(1):57-75 PMID: 4206504
  22. Apparent dispersive replication of yeast mitochondrial DNA as revealed by density labelling experiments.
    Mol Gen Genet. 1974;131(3):193-207 PMID: 4612326
  23. Maintenance of genetic homogeneity in systems with multiple genomes.
    Genet Res. 1976 Apr;27(2):249-65 PMID: 1278687
  24. Human malaria parasites in continuous culture.
    Science. 1976 Aug 20;193(4254):673-5 PMID: 781840
  25. Synchronization of Plasmodium falciparum erythrocytic stages in culture.
    J Parasitol. 1979 Jun;65(3):418-20 PMID: 383936
  26. S-antigens and isozymes in strains of Plasmodium falciparum.
    Trans R Soc Trop Med Hyg. 1981;75(6):855-8 PMID: 7036439
  27. Replication of animal mitochondrial DNA.
    Cell. 1982 Apr;28(4):693-705 PMID: 6178513
  28. Allelic dimorphism in a surface antigen gene of the malaria parasite Plasmodium falciparum.
    J Mol Biol. 1987 May 20;195(2):273-87 PMID: 3079521
  29. The localization of replication origins on ARS plasmids in S. cerevisiae.
    Cell. 1987 Nov 6;51(3):463-71 PMID: 2822257
  30. Prokaryotic DNA replication mechanisms.
    Philos Trans R Soc Lond B Biol Sci. 1987 Dec 15;317(1187):395-420 PMID: 2894677
  31. Purification of mature schizonts of Plasmodium falciparum on colloidal silica gradients.
    Bull World Health Organ. 1982;60(5):755-9 PMID: 6758971
  32. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  33. Yeast mitochondrial genomes consisting of only A.T base pairs replicate and exhibit suppressiveness.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):7156-60 PMID: 6390432
  34. The mitochondrion of Plasmodium falciparum visualized by rhodamine 123 fluorescence.
    J Protozool. 1985 Aug;32(3):442-6 PMID: 3900366
  35. In vivo homologous recombination intermediates of yeast mitochondrial DNA analyzed by electron microscopy.
    Mol Gen Genet. 1986 Mar;202(3):421-8 PMID: 3520238
  36. Tandemly arranged gene clusters of malarial parasites that are highly conserved and transcribed.
    Mol Biochem Parasitol. 1987 Jan 15;22(2-3):249-57 PMID: 3033497
  37. The extrachromosomal DNAs of apicomplexan parasites.
    Annu Rev Microbiol. 1994;48:81-104 PMID: 7826027
  38. The effect of (S)-9-(3-hydroxy-2-phosphonylmethoxypropyl) adenine on nuclear and organellar DNA synthesis in erythrocytic schizogony in malaria.
    Mol Biochem Parasitol. 1994 Sep;67(1):115-24 PMID: 7838172
  39. Mitochondrial DNA of the human malarial parasite Plasmodium falciparum.
    Mol Biochem Parasitol. 1988 Oct;31(1):11-7 PMID: 3054538
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-02-01
Pages
684-93
Language
English
Region
England
NLM ID
8208664
PMCID
PMC449987
Subset
IM
Grants
Medical Research Council · MC_U117532072 · United Kingdom
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2