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PMID: 7923355 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

DNA replication triggered by double-stranded breaks in E. coli: dependence on homologous recombination functions.

Cell ·Vol. 78 ·No. 6 ·1994-09-23 ·Pages 1051-61

Asai T, Bates DB, Kogoma T

Abstract

Homologous recombination-dependent DNA replication (RDR) of a lambda cos site-carrying plasmid is demonstrated in E. coli cells when the cells express lambda terminase that introduces a double-stranded break into the cos site. RDR occurs in normal wild-type cells if the plasmid also contains the recombination hotspot chi. Chi is dispensable when cells are induced for the SOS response or contain a recD mutation. recBC sbcA mutant cells are also capable of RDR induction. A recN mutation greatly reduces RDR in normal cells, but not in SOS-induced cells. RDR proceeds by the theta mode or rolling circle mode of DNA synthesis, yielding covalently closed circular plasmid monomers or linear plasmid multimers, respectively. Previously described inducible stable DNA replication is considered to be a special type of RDR that starts exclusively from specific sites (oriMs) on the chromosome.

Related Genes
recBC recD sbcA
MeSH Terms
Bacterial Proteins/genetics Base Sequence DNA Damage DNA Repair/genetics DNA Replication DNA Restriction Enzymes Endodeoxyribonucleases/genetics Escherichia coli/genetics Escherichia coli Proteins Exodeoxyribonuclease V Exodeoxyribonucleases/genetics Models, Genetic Molecular Sequence Data Mutation Plasmids/biosynthesis,genetics Recombination, Genetic SOS Response, Genetics Sequence Homology, Nucleic Acid
Chemicals
Bacterial Proteins Escherichia coli Proteins Endodeoxyribonucleases Exodeoxyribonucleases terminase Exodeoxyribonuclease V exodeoxyribonuclease V, E coli DNA Restriction Enzymes RecN protein, Bacteria
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Asai T
Department of Cell Biology, University of New Mexico School of Medicine, Albuquerque 87131.
Bates D B
Kogoma T
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/0092-8674(94)90279-8
Published
1994-09-23
Pages
1051-61
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC2988837
Subset
IM
Grants
NIGMS NIH HHS · R01 GM022092-22 · United States
NIGMS NIH HHS · GM22092 · United States
External Links
PubMed source DOI: 10.1016/0092-8674(94)90279-8 PMC Full Text View journal details
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