Home LiteratureArticle Details
PMID: 3298213 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Synthesis of linear multimers of OriC and pBR322 derivatives in Escherichia coli K-12: role of recombination and replication functions.

Journal of bacteriology ·Vol. 169 ·No. 7 ·1987-07-00 ·Pages 3131-7

Silberstein Z, Cohen A

Abstract

Inactivation of RecBCD nuclease (exonuclease V) and SbcB nuclease (exonuclease I) in Escherichia coli K-12 diverts most of plasmid replication activity from circular monomer production to the synthesis of linear multimers. Linear multimer synthesis has been demonstrated in plasmids of diverse origins and copy numbers, including E. coli minichromosomes. The effect of dnaA, dnaB, recF, and recJ mutations on the rate of linear multimer synthesis in sbcB cells after gam inactivation of RecBCD nuclease was investigated. Results are consistent with the hypothesis that homologous recombination, but not activities at the plasmid origin of replication, is involved in initiation of linear multimer synthesis.

MeSH Terms
Bacterial Proteins/physiology Chromosomes, Bacterial/physiology DNA Replication DNA, Bacterial/biosynthesis Escherichia coli/genetics Genes, Bacterial Plasmids Rec A Recombinases/physiology Recombination, Genetic
Chemicals
Bacterial Proteins DNA, Bacterial Rec A Recombinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Silberstein Z
Cohen A
References (38)
38 references, click to expand
  1. DNA replication: the rolling circle model.
    Cold Spring Harb Symp Quant Biol. 1968;33:473-84 PMID: 4891987
  2. A general priming system employing only dnaB protein and primase for DNA replication.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4308-12 PMID: 228295
  3. Replication of ColE1 plasmid deoxyribonucleic acid in a thermosensitive dnaA mutant of Escherichia coli.
    J Bacteriol. 1980 Mar;141(3):1024-30 PMID: 6988397
  4. Assay for type II restriction endonucleases using the Escherichia coli recBC DNase and duplex circular DNA.
    Methods Enzymol. 1980;65(1):26-8 PMID: 6246350
  5. Genetic and physical mapping of recF in Escherichia coli K-12.
    Mol Gen Genet. 1980;180(1):115-21 PMID: 6255290
  6. Genetic analysis of the RecE pathway of genetic recombination in Escherichia coli K-12.
    J Bacteriol. 1981 Jan;145(1):521-32 PMID: 6257642
  7. The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl-paper.
    Anal Biochem. 1980 Nov 15;109(1):123-9 PMID: 6162401
  8. In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals.
    J Bacteriol. 1980 Aug;143(2):971-80 PMID: 6162838
  9. Genetic recombination of bacterial plasmid DNA. Analysis of the effect of recombination-deficient mutations on plasmid recombination.
    J Mol Biol. 1982 Sep 25;160(3):411-30 PMID: 6759658
  10. Early intermediates in bacteriophage T4 DNA replication and recombination.
    J Virol. 1983 Feb;45(2):813-31 PMID: 6834472
  11. Plasmidic recombination in Escherichia coli K-12: the role of recF gene function.
    Mol Gen Genet. 1983;189(3):471-4 PMID: 6346018
  12. Rifampicin-induced replication of the plasmid pBR322 in Escherichia coli strains carrying dnaA mutations.
    Mol Gen Genet. 1983;190(2):326-30 PMID: 6308392
  13. Purified dnaA protein in initiation of replication at the Escherichia coli chromosomal origin of replication.
    Proc Natl Acad Sci U S A. 1983 Oct;80(19):5817-21 PMID: 6310593
  14. Genetic analysis of the recJ gene of Escherichia coli K-12.
    J Bacteriol. 1984 Jan;157(1):190-6 PMID: 6317649
  15. Control of ColE1 plasmid replication: the process of binding of RNA I to the primer transcript.
    Cell. 1984 Oct;38(3):861-70 PMID: 6207934
  16. Multiple origin usage for DNA replication in sdrA(rnh) mutants of Escherichia coli K-12. Initiation in the absence of oriC.
    J Mol Biol. 1984 Sep 15;178(2):227-36 PMID: 6387151
  17. Effect of dam methylation on the activity of the E. coli replication origin, oriC.
    EMBO J. 1985 May;4(5):1327-32 PMID: 3891330
  18. Genetic recombination of bacterial plasmid DNA: effect of RecF pathway mutations on plasmid recombination in Escherichia coli.
    J Bacteriol. 1985 Sep;163(3):1060-6 PMID: 2993230
  19. Identification and genetic analysis of sbcC mutations in commonly used recBC sbcB strains of Escherichia coli K-12.
    J Bacteriol. 1985 Nov;164(2):836-44 PMID: 3932331
  20. Intramolecular recombination of linear DNA catalyzed by the Escherichia coli RecE recombination system.
    J Mol Biol. 1985 Dec 5;186(3):515-25 PMID: 3005590
  21. The Escherichia coli dnaB replication protein is a DNA helicase.
    J Biol Chem. 1986 Apr 5;261(10):4738-48 PMID: 3007474
  22. Synthesis of linear plasmid multimers in Escherichia coli K-12.
    J Bacteriol. 1986 Jul;167(1):327-35 PMID: 3522548
  23. Selective inhibition of Escherichia coli recBC activities by plasmid-encoded GamS function of phage lambda.
    Gene. 1986;43(3):255-63 PMID: 2943636
  24. Hybridization between Escherichia coli and Shigella.
    J Bacteriol. 1957 Oct;74(4):461-76 PMID: 13475269
  25. Plasmid ColE1 DNA replication in Escherichia coli strains temperature-sensitive for DNA replication.
    Mol Gen Genet. 1975;136(4):273-89 PMID: 16094996
  26. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  27. Genetic recombination in Escherichia coli: the role of exonuclease I.
    Proc Natl Acad Sci U S A. 1971 Apr;68(4):824-7 PMID: 4927675
  28. Pedigrees of some mutant strains of Escherichia coli K-12.
    Bacteriol Rev. 1972 Dec;36(4):525-57 PMID: 4568763
  29. Recombination deficient mutants of E. coli and other bacteria.
    Annu Rev Genet. 1973;7:67-86 PMID: 4205905
  30. Detection of transcribable recombination products following conjugation in rec+, reCB- and recC-strains of Escherichia coli K12.
    J Mol Biol. 1974 Mar 15;83(4):447-57 PMID: 4598358
  31. Studies on the late replication of phage lambda: rolling-circle replication of the wild type and a partially suppressed strain, Oam29 Pam80.
    J Mol Biol. 1975 Oct 25;98(2):305-20 PMID: 1195390
  32. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  33. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  34. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  35. Replication of small plasmids in extracts of Escherichia coli: involvement of the dnaB and dnaC protein in the replication of early replicative intermediates.
    Mol Gen Genet. 1978 Jul 4;162(3):243-9 PMID: 355831
  36. Nucleotide sequence of the origin of replication of the Escherichia coli K-12 chromosome.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):580-4 PMID: 370833
  37. Origin of replication, oriC, of the Escherichia coli K12 chromosome: genetic mapping and minichromosome replication.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:121-8 PMID: 383376
  38. An Escherichia coli replication protein that recognizes a unique sequence within a hairpin region in phi X174 DNA.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):799-803 PMID: 6444725
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.7.3131-3137.1987
Published
1987-07-00
Pages
3131-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212360
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.169.7.3131-3137.1987 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2