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PMID: 6308392 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Rifampicin-induced replication of the plasmid pBR322 in Escherichia coli strains carrying dnaA mutations.

Molecular & general genetics : MGG ·Vol. 190 ·No. 2 ·1983-00-00 ·Pages 326-30

Polaczek P, Cieśla Z

Abstract

The replication pattern of the plasmid pBR322 was examined in the dnaA mutants of Escherichia coli. The rate of pBR322 DNA synthesis is markedly decreased after dnaA cells are shifted to the restrictive temperature of 42 degrees C. However, addition of rifampicin (RIF) to cultures of dnaA strains incubated at 42 degrees C after a lag of 90 min results in a burst of pBR322 synthesis. This RIF-induced pBR322 replication remains dependent on DNA polymerase I activity. Efficient plasmid pBR322 replication is observed at 42 degrees C in the double mutant dnaA46cos bearing an intragenic suppressor of dnaA46. Though replication of pBR322 in dnaA46cos growing at 42 degrees C is initially sensitive to RIF plasmid synthesis is restored after 90 min incubation in the presence of the drug. RIF-induced replication of the plasmid pBR327, lacking the rriB site implicated in RIF-resistant synthesis of the L strand of ColE1-like plasmids (Nomura and Ray 1981; Zipursky and Marians 1981), was observed also in dnaA46 at 42 degrees C.

MeSH Terms
Bacterial Proteins/genetics,physiology DNA Replication/drug effects DNA, Bacterial/biosynthesis Escherichia coli/enzymology,genetics Plasmids/drug effects Rifampin/pharmacology
Chemicals
Bacterial Proteins DNA, Bacterial Rifampin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Polaczek P
Cieśla Z
References (24)
24 references, click to expand
  1. A membrane-filter technique for the detection of complementary DNA.
    Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
  2. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  3. Escherichia coli factor Y sites of plasmid pBR322 can function as origins of DNA replication.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6111-5 PMID: 6273849
  4. Purification of the E. coli dnaA gene product.
    EMBO J. 1982;1(12):1545-9 PMID: 6327277
  5. The effects of an Escherichia coli dnaAts mutation on the replication of the plasmids colE1 pSC101, R100.1 and RTF-TC.
    Mol Gen Genet. 1979 Jul 13;174(2):117-26 PMID: 386040
  6. Suppression of the DnaA phenotype by mutations in the rpoB cistron of ribonucleic acid polymerase in Salmonella typhimurium and Escherichia coli.
    J Bacteriol. 1977 May;130(2):577-82 PMID: 400782
  7. Formation of an RNA primer for initiation of replication of ColE1 DNA by ribonuclease H.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2450-4 PMID: 6156450
  8. Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325.
    Gene. 1980 May;9(3-4):287-305 PMID: 6248430
  9. A cold sensitive dnaA mutant of E. coli which overinitiates chromosome replication at low temperature.
    Mol Gen Genet. 1978 Jun 1;162(1):9-16 PMID: 353526
  10. ColE1 plasmid mobility and relaxation complex.
    Nature. 1978 Jul 20;274(5668):259-61 PMID: 210387
  11. DNA polymerase as a requirement for the maintenance of the bacterial plasmid colicinogenic factor E1.
    Biochem Biophys Res Commun. 1970 Dec 24;41(6):1538-44 PMID: 4922634
  12. Replication of small plasmids in extracts of Escherichia coli.
    Mol Gen Genet. 1976 Jun 15;145(3):273-80 PMID: 781516
  13. Replication of ColE1 plasmid deoxyribonucleic acid in a thermosensitive dnaA mutant of Escherichia coli.
    J Bacteriol. 1980 Mar;141(3):1024-30 PMID: 6988397
  14. Plasmid cloning vehicles derived from plasmids ColE1, F, R6K, and RK2.
    Methods Enzymol. 1979;68:268-80 PMID: 232215
  15. Temporal sequence of events during the initiation process in Escherichia coli deoxyribonucleic acid replication: roles of the dnaA and dnaC gene products and ribonucleic acid polymerase.
    J Bacteriol. 1977 Mar;129(3):1466-75 PMID: 321429
  16. Plasmid ColE1 incompatibility determined by interaction of RNA I with primer transcript.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6096-100 PMID: 6171811
  17. Replication of small plasmids in extracts of Escherichia coli: involvement of the dnaB and dnaC protein in the replication of early replicative intermediates.
    Mol Gen Genet. 1978 Jul 4;162(3):243-9 PMID: 355831
  18. Determination of restriction sites and the nucleotide sequence surrounding the relaxation site of ColE1.
    J Mol Biol. 1978 Oct 5;124(4):601-39 PMID: 712849
  19. Two distinct mechanisms of synthesis of DNA fragments on colicin E1 plasmid DNA.
    Nature. 1975 Sep 18;257(5523):253-4 PMID: 1099459
  20. Relaxation complexes of plasmid DNA and protein. II. Characterization of the proteins associated with the unrelaxed and relaxed complexes of plasmid ColE1.
    J Biol Chem. 1975 Nov 25;250(22):8790-5 PMID: 1102544
  21. Isolation and characterization of amber mutations in gene dnaA of escherichia coli K-12.
    J Bacteriol. 1981 Feb;145(2):904-13 PMID: 7007354
  22. On the process of cellular division in Escherichia coli. 3. Thermosensitive mutants of Escherichia coli altered in the process of DNA initiation.
    J Mol Biol. 1970 Nov 14;53(3):369-87 PMID: 4924005
  23. Identification of two Escherichia coli factor Y effector sites near the origins of replication of the plasmids (ColE1 and pBR322.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6521-5 PMID: 6109282
  24. Identification of ColE1 DNA sequences that direct single strand-to-double strand conversion by a phi X174 type mechanism.
    Proc Natl Acad Sci U S A. 1982 May;79(10):3153-7 PMID: 6212928
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330659
Published
1983-00-00
Pages
326-30
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00330659
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