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PMID: 6212928 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of ColE1 DNA sequences that direct single strand-to-double strand conversion by a phi X174 type mechanism.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 10 ·1982-05-00 ·Pages 3153-7

Nomura N, Low RL, Ray DS

Abstract

A DNA single-strand initiation sequence, named rriA (called rri-1 previously), was detected in the origin region (Hae II fragment E) of the ColE1 plasmid [Nomura, N. & Ray, D. S. (1980) Proc. Natl. Acad. Sci. USA 77, 6566-6570]. Another site, called rriB, has been found on the opposite strand of Hae II fragment C. Both rriA and rriB (i) direct conversion of chimeric M13 phage single-stranded DNA to parental replicative form DNA in vivo by a rifampicin-resistant mechanism that is dependent on the dnaG and dnaB gene products, (ii) provide effector sites of dATP hydrolysis by primosomal protein n', and (iii) require the same primosomal proteins as phi X174 DNA for directing the in vitro conversion that rriA is the DNA sequence that determines the mechanism of lagging strand synthesis of ColE1 DNA and that the mechanism of discontinuous synthesis involves the primosomal proteins utilized in the in vitro conversion of phi X174 single strands to the double-stranded replicative form.

MeSH Terms
Bacterial Proteins/genetics Bacteriocin Plasmids Bacteriophage phi X 174/genetics DNA Replication DNA, Bacterial/genetics DNA, Single-Stranded/genetics Deoxyadenine Nucleotides/metabolism Genes Genes, Bacterial Plasmids
Chemicals
Bacterial Proteins DNA, Bacterial DNA, Single-Stranded Deoxyadenine Nucleotides
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nomura N
Low R L
Ray D S
References (26)
26 references, click to expand
  1. Replication of bacteriophage M13. VI. Attachment of M13 DNA to a fast-sedimenting host cell component.
    Virology. 1971 Mar;43(3):647-64 PMID: 4940969
  2. Replication of colicin E1 plasmid DNA in cell extracts. Origin and direction of replication.
    Proc Natl Acad Sci U S A. 1974 Jun;71(6):2260-4 PMID: 4601821
  3. Unidirectional replication of plasmid ColE1 DNA.
    Nature. 1974 Sep 27;251(5473):337-40 PMID: 4610399
  4. Relaxation complexes of plasmid DNA and protein. II. Characterization of the proteins associated with the unrelaxed and relaxed complexes of plasmid ColE1.
    J Biol Chem. 1975 Nov 25;250(22):8790-5 PMID: 1102544
  5. Cleavage map of colicin E1 plasmid.
    Nature. 1976 Nov 11;264(5582):193-6 PMID: 792714
  6. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  7. Origin of replication of colicin E1 plasmid DNA.
    Proc Natl Acad Sci U S A. 1977 May;74(5):1865-9 PMID: 325558
  8. The transposon Tn1 as a probe for studying ColE1 structure and function.
    Mol Gen Genet. 1977 Mar 7;151(2):151-60 PMID: 327263
  9. The RNA primer synthesized by primase to initiate phage G4 DNA replication.
    J Biol Chem. 1978 Feb 10;253(3):765-9 PMID: 340458
  10. ColE1 plasmid mobility and relaxation complex.
    Nature. 1978 Jul 20;274(5668):259-61 PMID: 210387
  11. Determination of restriction sites and the nucleotide sequence surrounding the relaxation site of ColE1.
    J Mol Biol. 1978 Oct 5;124(4):601-39 PMID: 712849
  12. Insertion mutant of bacteriophage f1 sensitive to EcoRI.
    Proc Natl Acad Sci U S A. 1979 Jun;76(6):2699-702 PMID: 379863
  13. Initiation of replication of plasmid ColE1 DNA by RNA polymerase, ribonuclease H, and DNA polymerase I.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:409-17 PMID: 225109
  14. Nucleotide sequence of small ColE1 derivatives: structure of the regions essential for autonomous replication and colicin E1 immunity.
    Mol Gen Genet. 1979 May 4;172(2):151-9 PMID: 384144
  15. A complementation analysis of mobilization deficient mutants of the plasmid ColE1.
    Mol Gen Genet. 1979 May 4;172(2):211-9 PMID: 225640
  16. Nucleotide sequence of the region required for maintenance of colicin E1 plasmid.
    Mol Gen Genet. 1979 Oct 3;176(2):161-70 PMID: 393952
  17. Cloning of a functional replication origin of phage G4 into the genome of phage M13.
    J Mol Biol. 1979 Dec 25;135(4):863-78 PMID: 231682
  18. An Escherichia coli replication protein that recognizes a unique sequence within a hairpin region in phi X174 DNA.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):799-803 PMID: 6444725
  19. Conjugal transmission of plasmids.
    Annu Rev Genet. 1979;13:99-125 PMID: 395897
  20. Formation of an RNA primer for initiation of replication of ColE1 DNA by ribonuclease H.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2450-4 PMID: 6156450
  21. Nucleotide sequence of the filamentous bacteriophage M13 DNA genome: comparison with phage fd.
    Gene. 1980 Oct;11(1-2):129-48 PMID: 6254849
  22. Identification of two Escherichia coli factor Y effector sites near the origins of replication of the plasmids (ColE1 and pBR322.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6521-5 PMID: 6109282
  23. Expression of a DNA strand initiation sequence of ColE1 plasmid in a single-stranded DNA phage.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6566-70 PMID: 7005899
  24. Construction and characterization of new coliphage M13 cloning vectors.
    Gene. 1980 Nov;11(3-4):207-18 PMID: 6260570
  25. Conservation of the primosome in successive stages of phi X174 DNA replication.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1436-40 PMID: 6262798
  26. Unique primed start of phage phi X174 DNA replication and mobility of the primosome in a direction opposite chain synthesis.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):69-73 PMID: 6454139
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.10.3153
Published
1982-05-00
Pages
3153-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346372
Subset
IM
Grants
NIAID NIH HHS · AI 10752-08 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.10.3153 PMC Full Text View journal details
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