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PMID: 8344265 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Homologous recombination-dependent initiation of DNA replication from DNA damage-inducible origins in Escherichia coli.

The EMBO journal ·Vol. 12 ·No. 8 ·1993-08-00 ·Pages 3287-95

Asai T, Sommer S, Bailone A, Kogoma T

Abstract

Escherichia coli cells induced for the SOS response express inducible stable DNA replication (iSDR) as an SOS function. Initiation of iSDR is independent of transcription, translation and DnaA protein, which are essential for initiation of DNA replication from oriC. We found that a recA mutant that is defective in recombination but proficient in SOS induction could not elicit iSDR. In contrast, iSDR was enhanced by recD and recJ mutations that inactivate the exonuclease V activity of the RecBCD enzyme and the RecJ exonuclease activity, respectively. A mutation in the ruvC gene that blocks the resolution of recombination intermediates (i.e. Holliday structures) also enhanced iSDR. Furthermore, inhibition of branch migration by recG or ruvAB mutations dramatically increased the iSDR activity. recBC mutants are defective in iSDR induction but the defect was suppressed by a mutation in the sbcA gene. The major product of minichromosomes replicated by iSDR was covalently closed circular monomers. We propose that recombination intermediates (i.e. D-loop structures) created by the action of RecA recombinase and RecBC(D) helicase play a central role in initiation of iSDR.

MeSH Terms
Bacterial Proteins/metabolism DNA Damage/genetics DNA Replication/genetics DNA, Bacterial/biosynthesis,genetics DNA, Circular/genetics Escherichia coli/genetics Escherichia coli Proteins Exodeoxyribonuclease V Exodeoxyribonucleases/metabolism Mutation Plasmids Rec A Recombinases/genetics,metabolism Recombination, Genetic SOS Response, Genetics
Chemicals
Bacterial Proteins DNA, Bacterial DNA, Circular Escherichia coli Proteins Rec A Recombinases Exodeoxyribonucleases RecJ protein, E coli recJ protein, Bacteria Exodeoxyribonuclease V exodeoxyribonuclease V, E coli
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Asai T
Department of Cell Biology, University of New Mexico School of Medicine, Albuquerque 87131.
Sommer S
Bailone A
Kogoma T
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-08-00
Pages
3287-95
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413596
Subset
IM
Grants
NIGMS NIH HHS · GM22092 · United States
External Links
PubMed source PMC Full Text View journal details
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